LC-DAD Determination of Fleroxacin in Bulk and Pharmaceutical Dosage Forms
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Data
2009-06-01
Autores
Salgado, Hérida Regina Nunes [UNESP]
Moreno, Andreia [UNESP]
Garg, Sanjay
Título da Revista
ISSN da Revista
Título de Volume
Editor
Vieweg
Resumo
Fleroxacin is a third generation fluoroquinolone with broad spectrum antibacterial activity. In this work an LC-DAD method for the analysis of fleroxacin was developed and validated using UV detection at 286 nm. The method was validated for linearity, precision, robustness, LOD, LOQ, specificity and accuracy at concentrations of 0.2-20.0 mu g mL(-1) and r (2) = 1. The LOD and LOQ were 0.059 and 0.197 mu g, respectively, the recoveries were 99.92-102.0% and the CV was less than 2.0%. The LC-DAD validated method provided analytical sensitivity, specificity and reproducibility suitable for quality control analysis.
Descrição
Palavras-chave
Column liquid chromatography, Liquid chromatography-diode array detection, Quality control, Fleroxacin
Como citar
Chromatographia. Wiesbaden: Vieweg, v. 69, p. 237-240, 2009.