Seletividade e absorção radicular do sulfentrazone em clones de eucalipto
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Data
2012-03-01
Autores
Carbonari, Caio Antonio [UNESP]
Velini, Edivaldo Domingues [UNESP]
Gomes, G.L.G.C. [UNESP]
Takahashi, E.N.
Araldi, R [UNESP]
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Editor
Sociedade Brasileira da Ciência das Plantas Daninhas
Resumo
O objetivo deste trabalho foi avaliar a seletividade e a absorção do sulfentrazone em clones de eucalipto. O primeiro experimento foi instalado em casa de vegetação, em delineamento inteiramente casualizado, com quatro repetições, no esquema fatorial 2 x 4, sendo duas doses do sulfentrazone (400 e 600 g ha-1) e quatro clones de eucalipto, híbridos de Eucalyptus grandis x E. urophylla (FB1, FB2, FB3 e FB4). Foram realizadas avaliações visuais de intoxicação das plantas de eucalipto e, no final do estudo, determinou-se a massa seca da parte aérea dos clones. No segundo experimento, foram utilizados os mesmos clones, sendo estes acondicionados em tubos falcon com 50 mL da solução contendo o sulfentrazone na concentração de 129 mM. As plantas de eucalipto permaneceram por 24 horas com as raízes imersas na solução e, em seguida, foi realizada a extração da seiva do xilema das plantas por meio de uma câmara de pressão. A concentração de sulfentrazone na seiva das plantas foi determinada através de cromatografia líquida e espectrometria de massas. O clone FB3 apresentou menor acúmulo de massa seca em relação aos demais, o que pode estar diretamente associado aos altos níveis de intoxicação observados. O clone FB2, apesar de mostrar elevada intoxicação, não apresentou níveis tão elevados de redução de massa seca em relação à testemunha. No tocante às concentrações de sulfentrazone nas plantas, elas foram proporcionais ao acúmulo de massa seca, o que indica que as variações na seletividade dos clones de eucalipto podem estar relacionadas à absorção diferenciada do herbicida.
The objective of this work was to evaluate the selectivity and absorption of sulfentrazone in clones of eucalyptus. The first experiment was set up in a greenhouse in a completely randomized design in a factorial 2 x 4, with two rates of sulfentrazone (400 and 600 g ha-1) and four clones of eucalyptus, hybrids of Eucalyptus grandis x E. urophylla (FB1, FB2, FB3, FB4), with four replications. Visual evaluations of intoxication of the eucalyptus plants were carried out and the dry mass of the plants was determined at the end of the study. In the second experiment, the same clones of eucalyptus were placed in falcon tubes with 50 mL of sulfentrazone at a concentration of 129 Mm, remaining for 24 hours until the extraction of xylem sap plant through a pressure pump. The concentration of sulfentrazone in the sap of the plants was determined by liquid chromatography and mass spectrometry. Clone FB3 showed greater dry mass reduction in relation to the other clones, and this reduction can be directly associated with the high levels of phytotoxicity observed. Clone FB2 presented high levels of intoxication, but did not show high dry mass reduction. The concentrations of sulfentrazone absorbed by the plants were proportional to the reductions in dry mass, indicating that variations in the selective eucalyptus clones may be related to the differentiated absorption of the herbicide.
The objective of this work was to evaluate the selectivity and absorption of sulfentrazone in clones of eucalyptus. The first experiment was set up in a greenhouse in a completely randomized design in a factorial 2 x 4, with two rates of sulfentrazone (400 and 600 g ha-1) and four clones of eucalyptus, hybrids of Eucalyptus grandis x E. urophylla (FB1, FB2, FB3, FB4), with four replications. Visual evaluations of intoxication of the eucalyptus plants were carried out and the dry mass of the plants was determined at the end of the study. In the second experiment, the same clones of eucalyptus were placed in falcon tubes with 50 mL of sulfentrazone at a concentration of 129 Mm, remaining for 24 hours until the extraction of xylem sap plant through a pressure pump. The concentration of sulfentrazone in the sap of the plants was determined by liquid chromatography and mass spectrometry. Clone FB3 showed greater dry mass reduction in relation to the other clones, and this reduction can be directly associated with the high levels of phytotoxicity observed. Clone FB2 presented high levels of intoxication, but did not show high dry mass reduction. The concentrations of sulfentrazone absorbed by the plants were proportional to the reductions in dry mass, indicating that variations in the selective eucalyptus clones may be related to the differentiated absorption of the herbicide.
Descrição
Palavras-chave
Eucalyptus spp., fitointoxicação, herbicida, Eucalyptus spp., phytointoxication, herbicide
Como citar
Planta Daninha. Sociedade Brasileira da Ciência das Plantas Daninhas , v. 30, n. 1, p. 147-153, 2012.