SISTEMAS QUIMILUMINESCENTES COM PEROXIDASE (EC:1.11.1.7) E SUAS APLICACOES EM ANALISES CLINICAS
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Data
1995-01-01
Autores
Brunetti, Iguatemy Lourenço [UNESP]
De Faria-Oliveira, O. M M [UNESP]
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Resumo
The enzyme horseradish peroxidase HRP (EC:1.11.1.7), has both acid and basic isoenzymes, catalyses a wide range of reactions (acting as an oxiredutase or an oxidase) and is thought capable of one- or two-electrons oxidations depending on the substrate employed. Today, the methodology for these assay can be chemiluminescent reactions and enhanced chemiluminescent. The enhanced chemiluminescent assay with system HRP, luminol, peroxide and an enhancer has provided the basis for a convenient and sensitive assay for peroxidase and peroxidase conjugates, DNA probe and blotting assay. It is particularly more advantageous than the others, because is very rapid, more sensitive (attomoles), easy to do and technically simple, and is relatively specific for HRP (reduces the effect of the interference).
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Palavras-chave
chemiluminescence, enhanced chemiluminescence, enzymes, luminol, peroxidase, horseradish peroxidase, peroxide, chemoluminescence, enzyme analysis, enzyme assay, review
Como citar
Revista de Ciencias Farmaceuticas, v. 16, p. 55-77.