Characteristics of the histamine release from hamster cheek pouch mast cells stimulated by lectins from Brazilian beans and concanavalin A
Data de publicação1996-10-10
Direito de acesso
MetadadosExibir registro completo
We have characterized the histamine releasing effects of lectins extracted from Brazilian beans, in comparison to concanavalin A, in hamster cheek pouch cell suspensions containing mast cells. The lectins from Dioclea virgata, Canavalia brasiliensis, and Dioclea rostrata induce histamine release in a similar manner to concanavalin A, but appear to differ in potency and efficacy. The effects depended on the temperature, pH, and metabolic energy, demonstrating the non-cytotoxic nature of the histamine release. It is suggested that the lectins studied act by the same mechanism as concanavalin A (interacting with sugars in the antibodies bound to the mast cells), since high concentrations of glucose inhibit the histamine release. The lectins at high concentrations quench the histamine release. This suppression is reversed by increasing calcium concentration, suggesting that the lectins bind to the calcium that is essential for the secretion, thereby confirming and extending our previous data using the lectin from Dioclea virgata in rat peritoneal mast cells.
Como citar este documento
Este item aparece nas seguintes coleções
Exibindo os itens relacionados pelo título, autor e palavra-chave.
The possible involvement of hyperpolarizing mechanisms in histamine-induced relaxation of the rat portal vein Rossignoli, Patrícia de S. ; Rodrigues, Andréa D.; Tinti, Thaís; Pereira, Oduvaldo C. M. ; Ellinger, Fred; Chies, Agnaldo B. (Journal of Smooth Muscle Research, 2008) [Artigo]The present study evaluated the effects of histamine 10 -2 M on longitudinal preparations of rat portal vein. It was observed that histamine 10 -2 M induced relaxation of rat portal vein preparations pre-contracted with ...
Gomes, J. C. (Agents and Actions, 1992) [Trabalho apresentado em evento]It is well known that histamine is found in high concentration in mast cell granules(1). The histamine content of these granules may be released to the extracellular space if an appropriate stimulus is provided(2). Besides ...
Effects of compound 48/80 on the guinea-pig isolated heart: A comparison with the in vitro cardiac anaphylaxis Gomes, J. C.; Antonio, A. (Pharmacological Research Communications, 1981) [Artigo]