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dc.contributor.authorFernandes, João Roberto [UNESP]
dc.contributor.authorDe Oliveira Neto, Graciliano
dc.contributor.authorKubota, Lauro T.
dc.contributor.authorTubino, Matthieu
dc.identifier.citationAnalytical Communications, v. 33, n. 11, p. 397-399, 1996.
dc.description.abstractThe enzyme oxalate oxidase, E.C. from Sorghum vulgare seeds (variety BR303) was used to develop a new sensor for oxalate determination without any purification. The sorghum seeds were conditioned in a 0.10 mol I-1 KCl solution. Then, these seeds were put in a stirring bar type enzymic reactor and coupled with an electrode for CO2. This device was introduced into a cell containing 10.0 ml of a 0.10 mol I-1 KCl solution saturated with oxygen. This sensor showed a linear response between 1.0 and 4.0 × 10-3 mol I-1 with a slope of 30 mV per decade of oxalate concentration at 25.0°C. The sensor was stable for one month or 200 determinations. The response time was about 60 s. The Michaelis-Menten constant determined for this enzyme was 1.5 × 10-3 mol I-1.en
dc.relation.ispartofAnalytical Communications
dc.titleUse of sorghum seed tissue as a biocatalyst in a stirred reactor for oxalic acid determinationen
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.description.affiliationDepartamento de Química FC UNESP, PO Box 473, 17033-360, Bauru, SP
dc.description.affiliationInstituto de Química Unicamp, PO Box 6154, 13083-970 Campinas, SP
dc.description.affiliationUnespDepartamento de Química FC UNESP, PO Box 473, 17033-360, Bauru, SP
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências, Baurupt
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