Application of HPLC-NMR coupling using C30 phase in the separation and identification of flavonoids of taxonomic relevance
Abstract
The on-line separation and identification of two important taxonomic markers for plant species of the Paepalanthus genus, the flavonoids 6-methoxykaempferol-3-O-β-D-glucopyranoside and 6-methoxykaempferol-3-O-β-D-6″(p-coumaroyl)glucopyranoside, has been performed with an HPLC-NMR coupling using C30 phase. 1D spectra have been recorded in the stopped-flow mode for the two predominant chromatographic peaks. This is the first application of HPLC-NMR coupling using C30 phase to a taxonomic problem. The technique drastically reduces the required amount of sampling for structure determination. © Springer-Verlag 2000.
How to cite this document
Santos, L. C. et al. Application of HPLC-NMR coupling using C30 phase in the separation and identification of flavonoids of taxonomic relevance. Fresenius' Journal of Analytical Chemistry, v. 368, n. 5, p. 540-542, 2000. Available at: <http://hdl.handle.net/11449/66291>.
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