Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck)
Nenhuma Miniatura disponível
Data
2000-12-01
Autores
Novelli, V. M. [UNESP]
Cristofani, M.
Machado, M. A.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Sweet orange is considered a very important species in the citrus world market and presents wide morphological variability. However, its characterization at the molecular level by random amplified polymorphic DNA (RAPD) and isozyme markers is not appropriate. Microsatellite or simple sequence repeats (SSRs) have been suggested as ideal for studies in cultures of vegetative propagation and as value markers for mapping in several species. However, information on microsatellite polymorphism in citrus species is scarce. In this work, microsatellite markers (AG-repeats) were developed from an enrichment library of genomic DNA of sweet orange cv. Pera (Citrus sinensis [L.] Osbeck), and 31 cultivars of sweet orange were evaluated. Evaluation of 18 microsatellite primers did not permit differentiation of the varieties studied. New microsatellite primers are being evaluated with the aim of detecting polymorphisms among the cultivars and closely related species to be used in genetic mapping programs.
Descrição
Palavras-chave
Breeding, Molecular markers, Polymerase chain reaction, Polymorphism
Como citar
Acta Horticulturae, v. 535, p. 47-50.