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dc.contributor.authorCeresini, Paulo C. [UNESP]
dc.contributor.authorDavid Shew, H.
dc.contributor.authorVilgalys, Rytas J. [UNESP]
dc.contributor.authorLiane Rosewich, U.
dc.contributor.authorCubeta, Marc A.
dc.date.accessioned2014-05-27T11:20:27Z
dc.date.available2014-05-27T11:20:27Z
dc.date.issued2002-05-01
dc.identifierhttp://www.mycologia.org/content/94/3/450.short
dc.identifierhttp://dx.doi.org/10.2307/3761779
dc.identifier.citationMycologia, v. 94, n. 3, p. 450-460, 2002.
dc.identifier.issn0027-5514
dc.identifier.urihttp://hdl.handle.net/11449/66885
dc.description.abstractA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to identify and differentiate genotypes of Rhizoctonia solani anastomosis group 3 subgroup PT (AG-3 PT), a fungal pathogen of potato. Polymorphic co-dominant single-locus PCR-RFLP markers were identified after sequencing of clones from a genomic library and digestion with restriction enzymes. Multilocus genotypes were determined by a combination of PCR product and digestion with a specific restriction enzyme for each of seven loci. A sample of 104 isolates from one commercial field in each of five counties in eastern North Carolina was analyzed, and evidence for high levels of gene flow between populations was revealed. When data were clone-corrected and samples pooled into one single North Carolina population, random associations of alleles were found for all loci or pairs of loci, indicating random mating. However, when all genotypes were analyzed, the observed genotypic diversity deviated from panmixia and alleles within and between loci were not randomly associated. These findings support a model of population structure for R. solani AG-3 PT on potato that includes both recombination and clonality.en
dc.format.extent450-460
dc.language.isoeng
dc.relation.ispartofMycologia
dc.sourceScopus
dc.subjectMigration
dc.subjectPopulation subdivision
dc.subjectThanatephorus cucumeris
dc.subjectdisease
dc.subjectgenetic structure
dc.subjectidentification method
dc.subjectpolymerase chain reaction
dc.subjectUnited States
dc.subjectHyphomycetes
dc.subjectMyxogastria
dc.subjectRhizoctonia
dc.subjectSolanum tuberosum
dc.titleGenetic structure of populations of Rhizoctonia solani AG-3 on potato in eastern North Carolinaen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionNorth Carolina State University
dc.contributor.institutionDuke University
dc.contributor.institutionUniversity of Minnesota
dc.description.affiliationDepartamento de Biologia Faculdade de Engenharia Universidade Estadual Paulista (UNESP), 15385-000, Ilha Solteira, Sao Paulo
dc.description.affiliationDepartment of Plant Pathology North Carolina State University, Raleigh, NC 27695
dc.description.affiliationDepartment of Biology Duke University, Durham, NC 27708
dc.description.affiliationUSDA-ARS Cereal Disease Laboratory University of Minnesota, Saint Paul, MN 55108
dc.description.affiliationUnespDepartamento de Biologia Faculdade de Engenharia Universidade Estadual Paulista (UNESP), 15385-000, Ilha Solteira, Sao Paulo
dc.identifier.doi10.2307/3761779
dc.identifier.wosWOS:000175866200010
dc.rights.accessRightsAcesso restrito
dc.identifier.scopus2-s2.0-0036588120
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Engenharia, Ilha Solteirapt
dc.identifier.file2-s2.0-0036588120.pdf
dc.relation.ispartofjcr2.762
dc.relation.ispartofsjr1,317
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