Structural, cytochemical, immunocytochemical and ultrastructural characterization of blood granulocytes of the roadside hawk Buteo magnirostris (Gmelin, 1788) (Avian, Falconiform)
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Data
2003-10-01
Autores
Santos, A. A.
Silva, A. Marques Joppert da
Regis Silva, M. R.
Cômodo Segreto, H. R.
Egami, M. Imoto
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Resumo
In the peripheral blood of the roadside hawk, Buteo magnirostris, the following types of granulocytic leucocytes were identified: heterophil, eosinophil and basophil. The heterophils presented acidophilic and spindle shaped granules, the eosinophils possess spherical eosinophilic granules and the basophils showed spherical and basophilic granules. The heterophils and eosinophils presented positive cytochemical reaction to glycogen and basic polyaminoacid, while the eosinophils presented sudanophilic granules, which were also positive for myeloperoxidase. The heterophils, alone, presented positivity for acid phosphatase in some granules and immunoreactivity to TGF-β1 was observed only in the cytoplasm of the eosinophils. Electron microscopy demonstrated the heterophil granules as predominantly spindle shaped, being strongly electron-dense, while the eosinophils had numerous uniformly electron-dense spherical granules and the basophils presented three different types of granules identified according to their electron-density and the aspect of their matrix.
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Palavras-chave
Avian, Cytochemistry, Granulocyte, Structure, TGFβ, Ultrastructure, acid phosphatase, cancer growth factor, glycogen, heterophile antibody, myeloperoxidase, polyaminoacid, tumor growth factor beta1, unclassified drug, animal cell, basophil, bird, blood, buteo magnirostis, cell density, controlled study, cytochemistry, cytoplasm, electron microscopy, eosinophil, granulocyte, hawk, immunocytochemistry, immunoreactivity, leukocyte, nonhuman, reaction analysis, structure analysis, ultrastructure, Animals, Basophils, Eosinophils, Granulocytes, Immunohistochemistry, Microscopy, Electron, Raptors, Transforming Growth Factor beta, Accipitrinae, Animalia, Aves, Buteo, Buteo magnirostris, Falconiformes
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Journal of Submicroscopic Cytology and Pathology, v. 35, n. 4, p. 351-357, 2003.