Morphometric study by image analysis of Ag-stained nucleoli in thyroids bearing proliferating lesions
MetadataShow full item record
The Ag-NOR staining technique and image analysis were used to evaluate morphological parameters (area, perimeter and axis ratio) in nucleoli from normal thyroids and from thyroids bearing proliferating lesions (carcinomas, adenomas and hyperplasias). Regions with normal appearance located close to adenomatous and carcinomatous regions, in the thyroid of every patient, were also analyzed for comparison with the respective pathological regions and with normal thyroids. Statistical analysis of data for the nucleolar area and perimeter allowed the separation of adenomas and carcinomas from hyperplasias and normal tissue but not the two components in each of these two groups. However, if we look at the numbers, a sequence of increasing nucleolar mean areas in the order: normal, hyperplasia, adenoma and carcinoma may be observed, indicating the sequence of increasing rRNA requirements in these different kinds of cells. The axis ratio that denotes the nucleolar shape (round or oblong) did not show significant differences among tissues, suggesting that shape is not important in the characterization of these pathologies. Differences in nucleolar areas and perimeter between normal and affected regions from each patient were statistically significant for adenomas and carcinomas. When these normal regions were compared with the normal thyroids, significant differences were not obtained in the three evaluated parameters. The observations and their importance for histopathological diagnosis are discussed.
How to cite this document
Showing items related by title, author, creator and subject.
Correlação anatomo-cintilográfica em 78 casos cirúrgicos de nódulos tireoidianos foliculares únicos benignos (adenomas) Santos, M. E.; Silva, W.; Andreghetti, C. Rezende (Revista do Hospital das Clinicas de Faculdade de Medicina da Universidade de Sao Paulo, 1981) [Artigo]
Chronic alcohol intake upregulates hepatic expression of carotenoid cleavage enzymes and PPAR in rats Luvizotto, Renata A. M.; Nascimento, André F.; Veeramachaneni, Sudipta; Liu, Chun; Wang, Xiang-Dong (Journal of Nutrition, 2010) [Artigo]Excessive and chronic alcohol intake leads to a lower hepatic vitamin A status by interfering with vitamin A metabolism. Dietary provitamin A carotenoids can be converted into vitamin A mainly by carotenoid 15,15′-monooxygenase ...
The importance of hormone receptor analysis in osteosarcoma cells growth submitted to treatment with estrogen in association with thyroid hormone Saraiva, Patricia Pinto; Teixeira, Silvania Silva; Conde, Sandro José ; Nogueira, Célia Regina (Cell Biochemistry and Function, 2008) [Artigo]Bone tumor incidence in women peaks at age 50-60, coinciding with the menopause. That estrogen (E2) and triiodothyronine (T3) interact in bone metabolism has been well established. However, few data on the action of these ...