Cytotoxicity of denture base resins: Effect of water bath and microwave postpolymerization heat treatments
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2004-05-01
Autores
Jorge, Janaina Habib [UNESP]
Giampaolo, Eunice Teresinha [UNESP]
Vergani, Carlos Eduardo [UNESP]
Machado, Ana Lucia [UNESP]
Pavarina, Ana Claudia [UNESP]
Carlos, Iracilda Zeppone [UNESP]
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Purpose: This study compared the effect of two postpolymerization heat treatments on the cytotoxicity of three denture base resins on L929 cells using 3H-thymidine incorporation and MTT assays. Materials and Methods: Sample disks of Lucitone 550, QC 20, and Acron MC resins were fabricated under aseptic conditions and stored in distilled water at 37°C for 48 hours. Specimens were then divided into three groups: (1) heat treated in microwave oven for 3 minutes at 500 W; (2) heat treated in water bath at 55°C for 60 minutes; and (3) no heat treatment. Eluates were prepared by placing three disks into a sterile glass vial with 9 mL of Eagle's medium and incubating at 37°C for 24 hours. The cytotoxic effect from the eluates was evaluated using the 3H-thymidine incorporation and MTT assays, which reflect DNA synthesis levels and cell metabolism, respectively. Results: The components leached from the resins were cytotoxic to L929 cells when 3H- thymidine incorporation assay was employed. In contrast, eluates from all resins revealed noncytotoxic effects as measured by MTT assay. For both MTT assay and 3H-thymidine incorporation, the heat treatments did not decrease the cytotoxicity of the materials tested. Conclusion: Resins were graded by 3H-thymidine incorporation assay as slightly cytotoxic and by MTT assay as noncytotoxic. Cytotoxicity of the denture base materials was not influenced by microwave or water bath heat treatment.
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resin, thymidine, animal cell, cell metabolism, controlled study, culture medium, cytotoxicity, denture, DNA synthesis, heat treatment, intermethod comparison, microwave radiation, mouse, nonhuman, polymerization, storage, temperature dependence, Acrylic Resins, Analysis of Variance, Animals, Coloring Agents, Denture Bases, Heat, L Cells (Cell Line), Mice, Mice, Inbred C3H, Microwaves, Molecular Structure, Phase Transition, Tetrazolium Salts, Tritium, Water
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International Journal of Prosthodontics, v. 17, n. 3, p. 340-344, 2004.