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  • ItemArtigo
    Dengue outbreaks in a city with recent transmission in São Paulo state, Brazil
    (2023-01-01) Prestes-Carneiro, Luiz Euribel; Barbosa Souza, Alana; Belussi, Gabriella Lima; Grande, Guilherme Henrique Dalaqua; Bertacco, Elaine Aparecida Maldonado; Vieira, André Gonçalves; Flores, Edilson Ferreira [UNESP]; Regional Hospital of Presidente Prudente; Municipal Epidemiological Surveillance of Presidente Prudente; Universidade Estadual Paulista (UNESP)
    We investigated the distribution of dengue cases, solid waste deposits (SWDs), forest fragments, water drainage, population income, and the possible association with dengue outbreaks in Presidente Prudente, São Paulo, Brazil. An urban setting with recent transmission. Data were obtained from public agencies. Kernel density maps of the variables were constructed. From 2015 to 2021, 33026 cases of dengue were reported; the incidence and mortality rate were highest in 2016. The number of cases decreased during the COVID-19 pandemic (2020 and 2021) compared with 2019, but alarming rates were registered in 2022. In 2015, 56 points of SWDs were identified, with an increase of 1.6-fold in 2020 and 2021. Multivariate analysis showed a positive correlation between the density of dengue cases and SWDs with the highest correlation (0.70) in 2020. Identifying these areas could guide public health authorities in surveillance measures and improvements in health care infrastructure.
  • ItemArtigo
    Bayesian analysis for multiple step-stress accelerated life test model under gamma lifetime distribution and type-II censoring
    (2023-03-23) Moala, Fernando Antonio [UNESP]; Chagas, Karlla Delalibera [UNESP]; Universidade Estadual Paulista (UNESP)
    Purpose: The step-stress accelerated test is the most appropriate statistical method to obtain information about the reliability of new products faster than would be possible if the product was left to fail in normal use. This paper presents the multiple step-stress accelerated life test using type-II censored data and assuming a cumulative exposure model. The authors propose a Bayesian inference with the lifetimes of test item under gamma distribution. The choice of the loss function is an essential part in the Bayesian estimation problems. Therefore, the Bayesian estimators for the parameters are obtained based on different loss functions and a comparison with the usual maximum likelihood (MLE) approach is carried out. Finally, an example is presented to illustrate the proposed procedure in this paper. Design/methodology/approach: A Bayesian inference is performed and the parameter estimators are obtained under symmetric and asymmetric loss functions. A sensitivity analysis of these Bayes and MLE estimators are presented by Monte Carlo simulation to verify if the Bayesian analysis is performed better. Findings: The authors demonstrated that Bayesian estimators give better results than MLE with respect to MSE and bias. The authors also consider three types of loss functions and they show that the most dominant estimator that had the smallest MSE and bias is the Bayesian under general entropy loss function followed closely by the Linex loss function. In this case, the use of a symmetric loss function as the SELF is inappropriate for the SSALT mainly with small data. Originality/value: Most of papers proposed in the literature present the estimation of SSALT through the MLE. In this paper, the authors developed a Bayesian analysis for the SSALT and discuss the procedures to obtain the Bayes estimators under symmetric and asymmetric loss functions. The choice of the loss function is an essential part in the Bayesian estimation problems.
  • ItemArtigo
    Dinaphtho[2,3-b:2′,3′-f]thieno[3,2-b]thiophene photoresistor: Generation of photocarriers and charge trapping
    (2023-10-01) Fernandes, José Diego [UNESP]; Vieira, Douglas Henrique [UNESP]; Klem, Maykel dos Santos [UNESP]; Alves, Neri [UNESP]; Universidade Estadual Paulista (UNESP)
    Thin films of organic semiconductors have been used as active layer in several electronic devices. The molecular organization of the active layer can be a critical parameter to optimize the performance of these devices. In this work, a thin film photoresistor of Dinaphtho[2,3-b:2′,3′-f]thieno[3,2-b]thiophene (DNTT) was fabricated using physical vapor deposition (PVD) technique with the most appropriate molecular organization to obtain better performance. The DNTT photoresistor current exhibited a significant increase under ultraviolet (UV) light (λ = 390 nm, Pd = 1.73 W/m2), achieving an IUV/IDark ratio of 1 × 105 at V = −1.5 V. The photoresistor DNTT achieved good values for the most important photodetector's figures of merit (FOMs), such as responsivity of 722.3 mA/W, detectivity of 1.0 × 1013 Jones and external quantum efficiency of 23000%. The measurements of real (Z′) and imaginary (Z″) impedance in the dark indicated that the electrical relaxation process is slow and of the Debye type. Nyquist plots revealed the appearance of negative capacitance (NC) at low frequencies when the photoresistor is under illumination, indicating that the photogenerated carriers can be trapped. The alternating current (AC) conductivity (σAC) measurements under lighting suggest that charge transfer occurs by hopping and those charge carriers can tunnel through the space charge region (SCR) with built-in potential generated by charges trapped. Therefore, the results showed that although the DNTT photoresistor has a good photoconductive response, its performance can be improved by reducing of traps.
  • ItemArtigo
    Perylene nanostructured films optimization for organic electronics: Molecular, structural, and electrochemical characterization
    (2023-07-31) Garcia, Melina P.B. [UNESP]; Martin, Cibely S. [UNESP]; Kavazoi, Henry S. [UNESP]; Maximino, Mateus D. [UNESP]; Alessio, Priscila [UNESP]; Universidade Estadual Paulista (UNESP); São Paulo Technology College (FATEC)
    The deposition technique, structure, and thermal treatment can influence the optical and electrical properties of perylene derivatives films. In this work, the nanostructured Langmuir-Schaefer films of bis(n-propylimide) perylene (PTCD-Pr) with thermal treatment were optimized for an electrochemical application. The PTCD-Pr film before and after thermal treatment was characterized by ultraviolet-visible absorption, infrared absorption spectroscopy, micro-Raman scattering, and voltammetry. Brewster angle microscopy images confirmed the higher aggregation degree of molecules on Langmuir film. The PTCD-Pr deposition showed a linear growth, forming H, J, and null aggregates. The infrared absorption spectroscopy indicates a preferential head-on orientation with a slight chromophore deviation. After thermal treatment (200 °C for 2 h), the PTCD-Pr film showed a decrease in the contribution of null aggregates while maintaining the same molecular orientation. The Raman and surface-enhanced Raman spectroscopy measurements also confirmed the presence of PTCD-Pr molecules distributed throughout the substrate surface, and the thermal treatment conditions did not degrade the PTCD-Pr molecule. Moreover, thermal treatment contributes to the increase of stability of the film at the electrode surface for electrochemical measurements. In the presence of active species (paraquat in this case), the thermally treated film showed promising electrocatalytic properties compared to the unmodified electrode.
  • ItemArtigo
    Integrating SPOCs in Software Testing Education: Evidence in Emergency Remote Courses
    (2023-01-01) Prates, Jorge Marques; Melo, Silvana Morita; Valle, Pedro Henrique Dias; Garcia, Rogério Eduardo [UNESP]; Maldonado, José Carlos; State University of Mato Grosso do Sul; Federal University of Grande Dourados; Federal University of Juiz de Fora; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)
    Nowadays, SPOCs (Small Private Online Courses) have been used as complementary methods to support classroom teaching. SPOCs are courses that apply the usage of MOOCs (Massive Open Online Courses), combining classroom with online education, making them an exciting alternative for contexts such as emergency remote teaching. Although SPOCs have been continuously proposed in the software engineering teaching area, it is crucial to assess their practical applicability via measuring the effectiveness of this resource in the teaching-learning process. In this context, this paper aims to present an experimental evaluation to investigate the applicability of a SPOC in a Verification, Validation, and Software Testing course taught during the period of emergency remote education during the COVID-19 pandemic in Brazil. Therefore, we conducted a controlled experiment comparing alternative teaching through the application of a SPOC with teaching carried out via lectures. The comparison between the teaching methods is made by analyzing the students’ performance during the solving of practical activities and essay questions on the content covered. In addition, we used questionnaires to analyze students’ motivation during the course. Study results indicate an improvement in both motivation and performance of students participating in SPOC, which corroborates its applicability to the software testing teaching area.
  • ItemArtigo
    Porphyrin-associated fluorescence spectroscopy (Photogen®) for the optical diagnosis of dental biofilm in orthodontic treatment: An observational clinical trial
    (2023-06-01) Ravazzi, Rodrigo; Neves, José Guilherme; Santamaria, Mauro Pedrine; Rosa, Luciano Pereira; Rosa, Francine Cristina Silva; Santamaria-Jr, Milton [UNESP]; SP; KY; Universidade Federal da Bahia (UFBA); Universidade Estadual Paulista (UNESP)
    Objective: : This study assessed the presence of dental biofilm with fluorescence spectroscopy associated with porphyrin (Photogen®) in users of orthodontic appliances. Methods: : This cross-sectional observational clinical trial included 21 patients with metallic orthodontic fixed appliances. The presence of biofilm was evaluated by fluorescence spectroscopy (Evince—MMÓptics. São Carlos-SP, Brazil) with a porphyrin photo-evidence device (Photogen®). Digital images of the buccal surface of the upper anterior teeth (central and lateral incisors and canines) without and with porphyrin were analyzed using the histogram R (red) function in ImageJ software. The results were analyzed using the maximum and mode values of the red pixels from the histograms. The statistical analysis considered the significance level of 5%. Results: : The maximum values and modes of the red pixels were significantly higher in biofilms analyzed by porphyrin-associated optical spectroscopy compared to optical spectroscopy alone. Conclusions: : Porphyrin-associated fluorescence spectroscopy was able to detect dental biofilm in the oral environment of patients with orthodontic treatment. This method better evidenced the presence of biofilm on the buccal surfaces of the upper teeth compared to that observed with fluorescence spectroscopy without porphyrin.
  • ItemArtigo
    Combining Impedance Spectroscopy and Information Visualization Methods to Optimize the Detection of Carbendazim Using Layer-by-Layer Films
    (2023-04-01) Furini, Leonardo Negri; Fernandes, José Diego [UNESP]; Vieira, Douglas Henrique [UNESP]; Morato, Luis Fernando do Carmo [UNESP]; Alves, Neri [UNESP]; Constantino, Carlos José Leopoldo [UNESP]; Universidade Federal de Santa Catarina (UFSC); Universidade Estadual Paulista (UNESP)
    Usually, electronic tongues (e-tongue) do not require specific interactions to discriminate aqueous solutions. Among the several factors which determine the electrical properties of sensing units, the interactions between liquids and interfaces have a crucial role. Here, we explore the interaction between dioctadecyldimethylammonium bromide (DODAB) lipid and carbendazim (MBC) pesticide in an e-tongue to discriminate different MBC concentrations in aqueous solutions. The sensing units were fabricated of gold interdigitated electrodes (IDEs) coated with layer-by-layer (LbL) films of DODAB and nickel tetrasulfonated phthalocyanine (NiTsPc), perylene and 1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DPPG), namely (DODAB/NiTsPc)5 and (Perylene/DPPG)5, respectively. Besides, a bare electrode also constituted the e-tongue to distinguish MBC concentrations from 1.0 × 10−7 up to 1.0 × 10−10 mol L−1, by impedance spectroscopy. In addition, the experiment was optimized using two IDE geometries. The LbL films were manually fabricated obtaining linear growth monitored via UV-Vis absorption spectroscopy. Optical images associated with chemical mapping reveals the presence of small aggregates in the DODAB/NiTsPc LbL film surface. Although the e-tongue was able to discriminate all MBC concentrations by means of the interactive document map (IDMAP), only the sensing unit covered with DODAB/NiTsPc LbL film presented a satisfactory response. According to the equivalent circuit, the main contribution arises from the bulk and film surface due to the interaction between DODAB and MBC, indicating THE sensitivity of the sensing unit. Finally, the adsorption of MBC molecules onto the film surface induced an irreversible process, although there are some frequencies at which the sensing unit response seems to be reversible, as shown by parallel coordinates.
  • ItemArtigo
    A multi-dimensional non-homogeneous Markov chain of order K to jointly study multi-pollutant exceedances
    (2023-01-01) Gallegos-Herrada, Marco A.; Rodrigues, Eliane R.; Tarumoto, Mario H. [UNESP]; Tzintzun, Guadalupe; Area de la Investigación Científica; Universidade Estadual Paulista (UNESP); Secretaría de Medio Ambiente y Recursos Naturales
    In this work we consider a multivariate non-homogeneous Markov chain of order K≥ 0 to study the occurrences of exceedances of environmental thresholds. In the model, d≥ 1 pollutants may be observed and, according to their respective environmental thresholds, a pollutant’s concentration measurement may be considered an exceedance or not. The parameters of the model are the order of the chain, and its initial and transition distributions. These parameters are estimated under the Bayesian point of view with the maximum a posteriori and leave-one-out cross validation methods used to estimate the order. In the case of the initial and transition probabilities, the estimation is made through samples generated using their respective posterior distributions. Once these parameters are obtained, we may estimate the probability of having no, one or more pollutants exceeding the associated environmental thresholds. This is made using the Markov property as well as a recurrence formula. Results are applied to the case where d= 2 which will correspond to ozone and particulate matter with diameter smaller than 10 microns (PM10) measurements obtained from the Mexico City monitoring network.
  • ItemResenha
    Waste-to-Energy Recovery from Municipal Solid Waste: Global Scenario and Prospects of Mass Burning Technology in Brazil
    (2023-03-01) Dadario, Natália [UNESP]; Gabriel Filho, Luís Roberto Almeida [UNESP]; Cremasco, Camila Pires [UNESP]; Santos, Felipe André dos [UNESP]; Rizk, Maria Cristina [UNESP]; Mollo Neto, Mario [UNESP]; Universidade Estadual Paulista (UNESP)
    Inadequate disposal of Municipal Solid Waste (MSW) is one of the greatest environmental issues confronted nowadays. One of the techniques used for its final disposal is incineration, otherwise known as mass burning. Although this procedure remains very controversial in Brazil, some recent studies published in Europe reveal a large amount of misinformation about it. It has been widely used in European countries, Japan, and a few U.S. cities and has been increasingly and significantly adopted in China. Therefore, this article aims to carry out a literature review on the evolution of waste-to-energy recovery from Municipal Solid Waste (MSW) worldwide and the progress of mass-burning technologies, particularly in the Brazilian context. For such a purpose, global scientific databases were selected and some of their results allowed us to present how the main WtE recovery technologies function, as well as their benefits and impacts. Moreover, it was possible to systematize the main regulatory frameworks on the theme in Brazil and to reveal the country’s electricity generation capacity, in addition to depicting the progress of Waste-to-Energy Plants (WtEPs) undergoing licensing processes in the state of São Paulo.
  • ItemArtigo
    Interrelationship between muscle fitness in childhood and bone mineral density in adulthood: mediation analysis of muscle fitness in adulthood
    (2023-12-01) Barbosa, Cynthia Correa Lopes; da Costa, Julio Cesar; Romanzini, Catiana Leila Possamai; Batista, Mariana Biagi; Blasquez-Shigaki, Gabriela; Fernandes, Rômulo Araújo [UNESP]; Martinho, Diogo V.; Oliveira, Tomás; Ribeiro, Luís P.; Coelho-e-Silva, Manuel João; Ronque, Enio Ricardo Vaz; Universidade Estadual de Londrina (UEL); Federal Technological University of Paraná - UTFPR; Universidade Federal de Mato Grosso do Sul (UFMS); Universidade Estadual Paulista (UNESP); University of Coimbra; University of Algarve
    Background: This study was aimed to examine the relationship between muscular fitness indicators in childhood and areal bone mineral density (aBMD) in adulthood and to verify whether the relationship is mediated by performance on muscular fitness indicators in adulthood. Methods: A sample of 138 healthy adults (69 males; 22.3 years) were followed after a previous assessment at the age of 7–10 years. Stature, body mass and muscular fitness indicators (handgrip strength, standing long jump and sit-ups tests) were assessed in childhood and adulthood. Additionally, total body, upper limbs, lower limbs, right femoral neck and lumbar spine aBMD was assessed in adulthood using dual X-ray absorptiometry. Analysis included descriptive statistics; t-test or Mann-Whitney U-test for comparison between males and females, multiple linear regression for the prediction aBMD from muscular fitness indicators in childhood, mediation analysis of the respective muscular fitness indicators in adulthood and the relationship between muscular fitness indicators in childhood and aBMD. Results: Males were stronger compared to females regarding muscular fitness indicators in childhood and adulthood, and presented higher mean values for aBMD in adulthood, except for lumbar spine (p < 0.05). Regression analysis revealed that some muscular fitness indicators in childhood showed significant positive relationship with bone health indicators in adulthood, such as: handgrip strength and total body aBMD (β = 0.005; R2 = 0.35; p = 0.040) and upper limbs aBMD (β = 0.005; R2 = 0.55; p = 0.019); and sit-ups test was a significant predictors of lumbar spine BMD (β = 0.003; R2 = 0.06; p = 0.039). Mediation analysis pointed out the following: adulthood handgrip strength mediated relationships between childhood handgrip strength and total aBMD (indirect effect (IE) = 0.0025; 95%CI = 0.0005–0.0048), and upper limbs aBMD (IE = 0.0040; 95%CI = 0.0017–0.0069). Conclusions: Muscular fitness indicators in childhood showed significant relationship with bone health indicators in adulthood and the sit-ups test in childhood had direct effect on lumbar spine aBMD in adulthood. Adulthood handgrip strength mediated the relationship between childhood handgrip strength and total body and upper limb aBMD, pointing out that muscular fitness in childhood may be a aBMD determinant in adulthood, especially when higher muscle fitness performance is maintained in adulthood.
  • ItemArtigo
    A performance comparison between honey and water as electrolytic dielectrics for ZnO liquid-gated transistors
    (2023-04-01) Vieira, Douglas H. [UNESP]; Nogueira, Gabriel L. [UNESP]; Ozório, Maíza S. [UNESP]; Fernandes, José D. [UNESP]; Seidel, Keli F.; Serbena, José P. M.; Alves, Neri [UNESP]; Universidade Estadual Paulista (UNESP); UTFPR; Universidade Federal do Paraná (UFPR)
    Liquid-gated transistors (LGTs) have attracted considerable attention due to their reduced fabrication time, low cost, and potential use in several applications, such as sensors for in loco diagnosis. Here, we conducted a comparative study of performance between honey-gated (HGT) and water-gated (WGT) transistors, using ZnO as the active layer, highlighting their main electrical characteristics and potential applications. The ZnO/honey and ZnO/water interfaces are attractive for edible and bioelectronics, as both materials are non-toxic and biocompatible. The mobility–capacitance product was determined from the transfer characteristics and found to be μsCi = 2.3 ± 0.8 μF V−1 s−1, and μsCi = 4.3 ± 0.5 μF V−1 s−1 for the WGT and HGT, respectively. Both devices exhibited non-null hysteresis, which was clockwise for the WGT and counterclockwise for the HGT. This phenomenon is linked to the liquid nature of the materials used as an electrolytic dielectric. The viscosity of honey results in lower ion mobility than water, leading to a higher |VTH| shift for the HGT. On the other hand, the WGT presents higher leakage current that dominates the off-state, while in the HGT, it is dominated by the channel resistance, resulting in a high Ion/Ioff ratio of (1.2 ± 0.7) × 104. The remarkable characteristics of the HGTs show that honey has potential as an organic gate dielectric for hybrid devices. With its low leakage current and high Ion/Ioff ratio at low-voltage operation, HGT presents an exciting opportunity as a platform to study material properties.
  • ItemCapítulo de livro
    Vibrational characterization methods for and with nanostructures
    (2020-01-01) Alessio, Priscila [UNESP]; Aoki, Pedro H.B. [UNESP]; Aroca, Ricardo F.; Universidade Estadual Paulista (UNESP); Faculty of Science
  • ItemArtigo
    Determination of the supramolecular arrangement of Dinaphtho[2,3-b:2′,3′-f]thieno[3,2-b]thiophene films fabricated by Physical Vapour Deposition and possible implications for electronic devices
    (2023-05-01) Fernandes, José Diego [UNESP]; Macedo, Wagner Costa; Vieira, Douglas Henrique [UNESP]; Furini, Leonardo Negri; Alves, Neri [UNESP]; Universidade Estadual Paulista (UNESP); Universidade Federal de Santa Catarina (UFSC)
    Organic thin films have been used as active layers and are an alternative to inorganic materials in order to improve the performance of electronic devices. Recently, Dinaphtho[2,3-b:2′,3′-f]thieno[3,2-b]thiophene (DNTT) became an important compound in organic electronics due to its optical and electrical properties, which are dependent on the supramolecular arrangement. Thus, in this work we determined the supramolecular arrangement of organic thin films of DNTT (DNTT PVD films) fabricated by Physical Vapour Deposition (PVD). Ultraviolet-visible absorption spectra showed the controlled growth of the films within the range from 17 to 120 nm and the formation of aggregates (J- and H-), as the H-aggregate was favored by the thickness. The emission spectra revealed that excimers are responsible for the emission in DNTT PVD films and the increase in solution concentration led to the formation of H-aggregates primarily. The molecular organization of the films, determined by selection rules of Fourier Transform Infrared absorption spectroscopy, suggested that the molecules had a preferential chain-on organization for 120 nm thickness film. Complementary, X-Ray Diffraction data showed that DNTT PVD film was practically monocrystalline with monoclinic cell unit, also determined for the 120 nm thickness film. Atomic Force Microscopy measurements revealed that DNTT film had a homogeneous surface (roughness around 6 nm for the film with 120 nm thickness) with grains of uniform size and geometry. Thus, based on the characteristics of the supramolecular arrangement (especially the molecular organization and increase of H-aggregates with the thickness of DNTT PVD film and the results found in the literature), it was possible to analyze probable implications of chain-on molecular organization and the increase of H-aggregates with the film thickness on the mobility of charge carriers of devices, such as diodes and transistors, in which they are applied.
  • ItemArtigo
    Zero Waste and Biodegradable Zinc Oxide Thin-Film Transistors for UV Sensors and Logic Circuits
    (2023-04-01) Nogueira, Gabriel L. [UNESP]; Kumar, Dinesh; Zhang, Shoushou; Alves, Neri [UNESP]; Kettle, Jeff; Universidade Estadual Paulista (UNESP); Bangor; James Watt School of Engineering
    Bioderived and biodegradable electronics have the capability to reduce significantly waste electrical and electronics equipment (WEEE) and can also be applied to other sectors, where degradation to benign by-products is essential, such as marine, farming, or health monitoring. Herein, the authors report biodegradable thin-film transistors (TFTs) arrays based on zinc oxide (ZnO) active layer using molybdenum (Mo) source, drain, and gate electrodes. The developed TFTs were fabricated at room temperature onto a planarized biodegradable substrate surface and achieved an Ion/Ioff ratio of ∼ 4× 106 , a threshold voltage of ∼ 2.3 V, a field-effect mobility in the saturation region of 1.3 cm 2 V-1 s-1 , and a subthreshold swing of 0.3 V dec -1 and show stable device performance under stability tests. Based upon the successful fabrication of the ZnO TFT array, the demonstration of a UV sensor (phototransistors mode) and simple logic circuits (inverter and both NAND and NOR gate circuits) are presented. Furthermore, a method to 'control' the transience was implemented by using a printed heater that could accelerate the decomposition of material, which opens potential avenue for material recovery and zero waste products.
  • ItemArtigo
    Synthesize of pluronic-based nanovesicular formulation loaded with Pistacia atlantica extract for improved antimicrobial efficiency
    (2023-06-01) Al-Ouqaili, Mushtak T.S.; Saleh, Raed Obaid; Amin, Hawraz Ibrahim M.; Jawhar, Zanko Hassan; Akbarizadeh, Majid Reza; Naderifar, Mahin; Issa, Kovan Dilawer; Gavilán, Juan Carlos Orosco; Nobre, Marcos Augusto Lima [UNESP]; Jalil, Abduladheem Turki; Akhavan-Sigari, Reza; University of Anbar; Al-Maarif University College; Salahaddin University-Erbil; Cihan University-Erbil; Lebanese French University; Hawler Medical University; Zabol University of Medical Sciences; Tishk International University; Universidad Privada del Norte; Universidade Estadual Paulista (UNESP); Al-Mustaqbal University College; University Medical Center Tuebingen; Collegium Humanum Warsaw Management University
    One of the current concerns to human health is antibiotic resistance, which promotes the use of antibiotics that are more harmful, expensive, and ineffective. In this condition, researchers are turning to innovative options to combat this alarming situation. Combining herbal medicine with nanotechnology has created a new strategy to increase the effectiveness of phytochemical compounds in overcoming antimicrobial resistance. Pistacia atlantica is one of the promising herbs with medicinal benefits, but its poor solubility in biological fluids is challenging. In this regard, we seek to evaluate the antibacterial efficacy of Pistacia atlantica extract-loaded nanovesicle. Cholesterol, Span 40, and Pluronic F127 modified nanoformulation was developed using an environmentally friendly improved heating technique, and it was evaluated for size distribution, zeta potential, morphology, entrapment efficiency (EE%), release behavior, stability, and antimicrobial performance. By using DLS, spherical nanovesicles were identified with a size distribution of 50–150 nm and a zeta potential of −43 mV. The extract's encapsulation efficiency was 72.03%. The developed loaded nanovesicles demonstrated controlled extract release in the tested 96 h and storage stability of at least 12 months at 25 °C. Also, Comparing the two samples, the encapsulated extract had greater antibacterial activity against Candida albicans, Staphylococcus aureus, and Pseudomonas plecoglossicida with MIC of 1320, 570, and 1100 µg/mL, respectively. Besides reducing the misuse of antibiotics by allowing for the controlled release of drugs made from natural sources, we expect the findings described here to help provide alternative plant-based formulations with greater stability and antibacterial activity.
  • ItemArtigo
    Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum
    (2023-05-01) Castro, Karla R.; Setti, Grazielle O. [UNESP]; de Oliveira, Tássia R.; Rodrigues-Jesus, Mônica J.; Botosso, Viviane F.; Perini de Araujo, Ana P.; Durigon, Edson L.; Ferreira, Luis C.S.; Faria, Ronaldo C.; Universidade Federal de São Carlos (UFSCar); Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP); Butantan Institute
    Zika virus (ZIKV) is a flavivirus transmitted by infected Aedes genus mosquitoes. An infected person may be asymptomatic or present symptoms such as fever, arthralgia, and in pregnancy it may lead to neurological disorders in the fetus, such as microcephaly. Based on the high dissemination potential of ZIVK and its similar antigen composition to other arboviruses, new approaches for selective virus detection are urgently needed. This work reports the development of an electrochemical immunoassay for detection of anti-ZIKV antibodies, using magnetic beads functionalized with recombinant protein derived from the non-structural protein 1 (ΔNS1-ZIKV) and anti-IgG antibodies labeled with horseradish peroxidase (HRP) enzyme. The magneto-immunoassay uses disposable microfluidic devices for detection of anti-ZIKV in serum samples. A linear response was obtained for a wide concentration range from 0.01 to 9.80 × 105 pg mL−1 (r2 = 0.982), with a limit of detection of 0.48 pg mL−1. The proposed immunoassay proved to be highly efficient for the detection of anti-ZIKV antibodies in serum, offering promising perspectives for the development of fast, simple, and affordable point-of-care diagnosis devices for ZIKV.
  • ItemArtigo
    Objective Prior Distributions to Estimate the Parameters of the Poisson-Exponential Distribution
    (2023-01-01) Moala, Fernando A. [UNESP]; Moraes, Gustavo [UNESP]; Universidade Estadual Paulista (UNESP)
    In this paper, a set of important objective priors are examined for the Bayesian estimation of the parameters present in the Poisson-Exponential distribution P E. We derived the multivariate Jeffreys prior and the Maximal Data Information Prior. Reference prior and others priors proposed in the literature are also analyzed. We show that the posterior densities resulting from these approaches are proper although the respective priors are improper. Monte Carlo simulations are used to compare the efficiencies and to assess the sensitivity of the choice of the priors, mainly for small sample sizes. This simulation study shows that the mean square error, mean bias and coverage probability of credible intervals under Gamma, Jeffreys' rule and Box & Tiao priors presented equal results, whereas Jeffreys and Reference priors showed the best results. The MDIP prior had a worse performance in all analyzed situations showing not to be indicated for Bayesian analysis of the P E distribution. A real data set is analyzed for illustrative purpose of the Bayesian approaches.
  • ItemArtigo
    Natural rubber/wood composite foam: Thermal insulation and acoustic isolation materials for construction
    (2023-03-01) Neves de Alencar, Laura [UNESP]; Guedes Paiva, Fábio Friol; Okimoto, Fernando Sérgio [UNESP]; Bacarin, Giovani Boaventura [UNESP]; Dognani, Guilherme [UNESP]; Salmazo, Leandra Oliveira; dos Santos, Renivaldo José [UNESP]; Cabrera, Flávio Camargo [UNESP]; Job, Aldo Eloizo [UNESP]; Universidade Estadual Paulista (UNESP); University of Western São Paulo (UNOESTE); University of Valladolid (UVa)
    With the advances in the field of civil construction and the world population growth, the development of policies is necessary for the management and reuse of generated residue. Thus, the present work proposes the use of expanded natural rubber as a polymeric matrix incorporated with eucalyptus filler as a reinforcing filler for the production of composites. Thermal insulation capacity was determined by the transient plane source and acoustic method by impedance tube. NR/W40 foam showed enhanced the acoustic insulation capacity. The maximum absorption of NR/W40 was 0.83, at 3257 Hz, which is three times higher than natural rubber foam. Highly inhomogeneous cell structures were observed with large, interconnected pores, improving the acoustic performance. Sound absorption coefficient for natural rubber foam with 40% wood (0.83 ± 0.046) was similar to PU foam (0.97 ± 0.009) with 20 mm in thickness, a density of 47 kg/m3 and 98% open cell content it is a well-known acoustic absorbent in the building sector. The NR/W40 sample recorded the best acoustic performance among the NR foams analyzed in this work, maintaining good sound absorption above 1500 Hz, demonstrating a possibility of wood reuse as a filler in based-rubber foam for acustic insulation.
  • ItemArtigo
    Effect of phenolic resin content in waste foundry sand on mechanical properties of cement mortars and leaching of phenols behaviour
    (2023-04-01) de Paiva, Fábio Friol Guedes; dos Santos, Luis Fernando [UNESP]; Tamashiro, Jacqueline Roberta; Pereira Silva, Lucas Henrique; Teixeira, Silvio Rainho [UNESP]; Galvín, Adela P.; López-Uceda, Antonio; Kinoshita, Angela; UNOESTE; Universidade Estadual Paulista (UNESP); Leonardo da Vinci – Campus of Rabanales
    Waste foundry sand (WFS) is usually a sand substitute in cementitious materials. However, some of them are contaminated with phenolic resin (WFPS) and there are few papers about it, due to difficulties to reuse. This work focuses on mortar with WFPS as a candidate to immobilize phenolic resin and their environmental safety, since phenols are hazardous materials. Two different WFPS, with low and high resin contents, and a third, with alkaline treatment, were studied. Mortar with WFPS with low resin content reached satisfactory mechanical strength while with the high resin content presented a reduction of up to 50%. The leaching of phenols by WFPS with high resin contents reached up to 20 times the normative limit. However, the leaching of phenols of mortar, in the monolithic state, in long-term is within the limits, demonstrating the possibility of recycling this waste produced in large quantities worldwide by foundry industries.
  • ItemArtigo
    Chitosan and HPMCAS double-coating as protective systems for alginate microparticles loaded with Ctx(Ile21)-Ha antimicrobial peptide to prevent intestinal infections
    (2023-02-01) Roque-Borda, Cesar Augusto [UNESP]; Saraiva, Mauro de Mesquita Souza [UNESP]; Macedo Junior, Wagner Dias [UNESP]; Márquez Montesinos, José Carlos Estanislao; Meneguin, Andréia Bagliotti [UNESP]; Toledo Borges, Anna Beatriz [UNESP]; Crusca Junior, Edson [UNESP]; Garrido, Saulo Santesso [UNESP]; de Almeida, Adriana Maria [UNESP]; Marchetto, Reinaldo [UNESP]; Chorilli, Marlus [UNESP]; Berchieri Junior, Angelo [UNESP]; Teixeira, Silvio Rainho [UNESP]; Pavan, Fernando Rogério [UNESP]; Vicente, Eduardo Festozo [UNESP]; Universidade Estadual Paulista (UNESP); Universidad de Talca
    The incorrect use of conventional drugs for both prevention and control of intestinal infections has contributed to a significant spread of bacterial resistance. In this way, studies that promote their replacement are a priority. In the last decade, the use of antimicrobial peptides (AMP), especially Ctx(Ile21)-Ha AMP, has gained strength, demonstrating efficient antimicrobial activity (AA) against pathogens, including multidrug-resistant bacteria. However, gastrointestinal degradation does not allow its direct oral application. In this research, double-coating systems using alginate microparticles loaded with Ctx(Ile21)-Ha peptide were designed, and in vitro release assays simulating the gastrointestinal tract were evaluated. Also, the AA against Salmonella spp. and Escherichia coli was examined. The results showed the physicochemical stability of Ctx(Ile21)-Ha peptide in the system and its potent antimicrobial activity. In addition, the combination of HPMCAS and chitosan as a gastric protection system can be promising for peptide carriers or other low pH-sensitive molecules, adequately released in the intestine. In conclusion, the coated systems employed in this study can improve the formulation of new foods or biopharmaceutical products for specific application against intestinal pathogens in animal production or, possibly, in the near future, in human health.