Evaluation of homologous, heterologous, and affinity conjugates for the serodiagnosis of Toxoplasma gondii and Neospora caninum in maned wolves (Chrysocyon brachyurus)
MetadataShow full item record
Use of serological tests in the diagnosis of infectious diseases in wild animals has several limitations, primarily the difficulty of obtaining species-specific reagents. Wild canids, such as maned wolves (Chrysocyon brachyurus), are highly predisposed to infection by Toxoplasma gondii and, to a lesser extent, to Neospora caninum. The aim of the present study was to evaluate homologous, heterologous, and affinity conjugates in enzyme-linked immunosorbent assays (ELISAs) and indirect fluorescent antibody tests (IFATs) for detecting immunoglobulin (Ig) G antibodies against T. gondii and N. caninum in maned wolves. Serum samples were obtained from 59 captive animals in Brazil and tested by ELISA for T. gondii serology and IFAT for N. caninum serology using 3 different enzymatic and fluorescent conjugates: homologous (guinea pig anti-maned wolf IgG-peroxidase and -fluorescein isothiocyanate [FITC]), heterologous (rabbit anti-dog IgG-peroxidase and -FITC), and affinity (protein A-peroxidase and -FITC). Seropositivity to T. gondii was comparable among the homologous (69.5%), heterologous (74.6%), and affinity (71.2%) enzymatic conjugates. A significant positive correlation was found between the antibody levels determined by the 3 enzymatic conjugates. The highest mean antibody levels (ELISA index = 4.5) were observed with the protein A-peroxidase conjugate. The same seropositivity to N. caninum (8.5%) was found with the homologous and heterologous fluorescent conjugates, but protein A-FITC was not able to detect or confirm any positive samples with homologous or heterologous conjugates. Our results demonstrate that homologous, heterologous, and affinity conjugates might be used in ELISA for serological assays of T. gondii in wild canids, whereas for N. caninum infection, only the homologous or heterologous fluorescent conjugates have been shown to be useful. © American Society of Parasitologists 2005.
How to cite this document
Showing items related by title, author, creator and subject.
Toxoplasma gondii: Comparison of a rhoptry-ELISA with IFAT and MAT for antibody detection in sera of experimentally infected pigs Garcia, João Luis; Navarro, Italmar Teodorico; Vidotto, Odilon; Gennari, Solange Maria; Machado, Rosângela Zacarias ; Pereira, Ademir Benedito da Luz; Sinhorini, Idercio Luiz (Experimental Parasitology, 2006) [Artigo]Indirect ELISA and IFAT have been reported to be more sensitive and specific than agglutination tests. However, MAT is cheaper, easier than the others and does not need special equipment. The purpose of this study was to ...
Victor Canettieri, Antonio Carlos ; Kretchetoff, Fujiko Yamasiro; Koga Ito, Cristiane Yumi ; Moreira, Daniella ; Condino Fujarra, Fabio José; Unterkircher, Carmelinda Schmidt (Brazilian Oral Research, 2006) [Artigo]Several studies have been conducted in the last decades aiming to obtain an anti-canies vaccine, however some studies have demonstrated cross reactivity between Streptococcus mutans surface antigens and the human cardiac ...
Autoimmunity does not contribute to the highly prevalent glucose metabolism disturbances in a Japanese Brazilian population Hashimoto, Silvia M.; Hirai, A. T.; Kikuchi, M.; Cardoso, M. A. ; Tomita, N.; Chaim, R.; Wakisaka, K.; Gimeno, Suely G.A.; Matsumura, Luiza; Franco, Laercio J. et al. (Ethnicity and Disease, 2007) [Artigo]The Japanese Brazilian population has one of the highest prevalences of diabetes worldwide. Despite being non-obese according to standard definitions, their body fat distribution is typically central. We investigated whether ...