Monomolecular films of cholesterol oxidase and S-Layer proteins
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Data
2011-05-15
Autores
Ferraz, Helen Conceicao
Guimaraes, Juliana Aguilar
Moitinho Alves, Tito Livio
Leopoldo Constantino, Carlos Jose [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Cholesterol oxidase (ChOx) is a flavoenzyme that catalyzes the oxidation of cholesterol to cholest-5-en-3-one and subsequently the isomerization to cholest-4-en-3-one. ChOx has been very commonly studied as the detection element in cholesterol biosensors. In the biosensor development field, a relatively new approach is the use of crystalline bacterial cell surface layers, known as S-Layer proteins. These proteins exhibit the ability of self-assembling at surfaces, opening a vast spectrum of applications, both in basic and applied researches. In our study, monomolecular films of ChOx and mixed films of ChOx/S-Layer proteins and DPPC/S-Layer proteins were produced using the Langmuir technique. Characterization of the films was performed by means of surface pressure-molecular area (pi-A) isotherms. Stable monolayers were obtained, which means that they can be transferred to solid substrates by Langmuir-Blodgett technique. Mixed monolayers showed an ideal like behavior. (C) 2011 Elsevier B. V. All rights reserved.
Descrição
Palavras-chave
Cholesterol, Cholesterol oxidase, S-Layer proteins, Monolayer, Biosensor
Como citar
Applied Surface Science. Amsterdam: Elsevier B.V., v. 257, n. 15, p. 6535-6539, 2011.