Effect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell line

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2006-09-01

Autores

Aranha, Andreza M.F. [UNESP]
Giro, Elisa M.A. [UNESP]
Souza, Pedro P.C. [UNESP]
Hebling, Josimeri [UNESP]
de Souza Costa, Carlos A. [UNESP]

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Resumo

Objective: The aim of this in vitro study was to evaluate the cytotoxicity of resin-modified glass-ionomer lining cements submitted to different curing regimes and applied to an immortalized odontoblast-cell line (MDPC-23). Methods: Forty round-shaped specimens of each experimental material (Fuji Lining LC and Vitrebond) were prepared. They were light-cured for the manufacturers' recommended time (MRT = 30 s), under-cured (0.5 MRT = 15 s), over-cured (1.5 MRT = 45 s) or allowed to dark cure (0 MRT). Sterilized filter papers soaked with either 5 μL of PBS or HEMA were used as negative and positive control, respectively. After placing the specimens individually in wells of 24-well dishes, odontoblast-like cells MDPC-23 (30,000 cells/cm2) were plated in each well and incubated for 72 h in a humidified incubator at 37 °C with 5% CO2 and 95% air. The cytotoxicity was evaluated by the cell metabolism (MTT assay) and cell morphology (SEM). Results: Fuji Lining LC was less cytotoxic than Vitrebond (p < 0.05) in all the experimental conditions. However, the cytotoxicity of Fuji Lining LC was noticeably increased in the absence of light-curing while the same was not observed for Vitrebond. The length of light-curing (15, 30 or 45 s) did not influence the toxicity of both lining materials when they were applied on the odontoblast-cell line MDPC-23. Significance: The light-activation plays an important role in reducing the cytotoxicity of Fuji Lining LC. Following the manufacturer' recommendation regarding the light-curing regime may prevent toxic effect to the pulp cells. © 2005 Academy of Dental Materials.

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Curing regime, Cytotoxicity, Glass-ionomer cements, HEMA, Odontoblast, Cell culture, Chemical bonds, Curing, Glass, Ionomers, Morphology, Dental cement, 2 hydroxyethyl methacrylate, 4 anisyltetrazolium blue, 4-anisyltetrazolium blue, coloring agent, dentin bonding agent, Fuji glass ionomer lining cement, Fuji glass-ionomer lining cement, glass ionomer, methacrylic acid derivative, resin, tetrazolium, Vitrabond, animal, cell line, cell shape, chemistry, dental surgery, drug effect, light, metabolism, mouse, odontoblast, phase transition, radiation exposure, scanning electron microscopy, Animals, Cell Line, Transformed, Cell Shape, Coloring Agents, Dental Cavity Lining, Dentin-Bonding Agents, Glass Ionomer Cements, Light, Methacrylates, Mice, Microscopy, Electron, Scanning, Odontoblasts, Phase Transition, Resins, Synthetic, Tetrazolium Salts

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Dental Materials, v. 22, n. 9, p. 864-869, 2006.