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dc.contributor.authorBravo, Juliana Pereira [UNESP]
dc.contributor.authorHoshino, Andrea Akemi [UNESP]
dc.contributor.authorAngelici, Carla Maria Lara C.D. [UNESP]
dc.contributor.authorLopes, Catalina Romero [UNESP]
dc.contributor.authorGimenes, Marcos Aparecido [UNESP]
dc.date.accessioned2014-05-27T11:21:58Z
dc.date.available2014-05-27T11:21:58Z
dc.date.issued2006-09-20
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572006000300021
dc.identifier.citationGenetics and Molecular Biology, v. 29, n. 3, p. 516-524, 2006.
dc.identifier.issn1415-4757
dc.identifier.issn1678-4685
dc.identifier.urihttp://hdl.handle.net/11449/69094
dc.description.abstractThe Arachis section is the most important of the nine sections of the genus Arachis because it includes the cultivated peanut, Arachis hypogaea. The genetic improvement of A. hypogaea using wild relatives is at an early stage of development in spite of their potential as sources of genes, including those for disease and pests resistance, that are not found in the A. hypogaea primary gene pool. Section Arachis species germplasm has been collected and maintained in gene banks and its use and effective conservation depends on our knowledge of the genetic variability contained in this material. Microsatellites are routinely used for the analysis of genetic variability because they are highly polymorphic and codominant. The objective of this study was to evaluate the transferability of microsatellite primers and the assay of genetic variability between and within the germplasm of some species of the Arachis section. Fourteen microsatellite loci developed for three different species of Arachis were analyzed and 11 (78%) were found to be polymorphic. All loci had transferability to all the species analyzed. The polymorphic loci were very informative, with expected heterozygosity per locus ranging from 0.70 to 0.94. In general, the germplasm analyzed showed wide genetic variation. © 2006 Sociedade Brasileira de Genética.en
dc.format.extent516-524
dc.language.isoeng
dc.relation.ispartofGenetics and Molecular Biology
dc.sourceScopus
dc.subjectGenetic diversity
dc.subjectGermplasm
dc.subjectPeanut
dc.subjectTransferability
dc.subjectWild species
dc.subjectcodominance
dc.subjectdisease resistance
dc.subjectDNA extraction
dc.subjectgene amplification
dc.subjectgene locus
dc.subjectgene pool
dc.subjectgene transfer
dc.subjectgenetic analysis
dc.subjectgenetic conservation
dc.subjectgenetic improvement
dc.subjectgenetic polymorphism
dc.subjectgenetic variability
dc.subjectgermplasm
dc.subjectheterozygosity
dc.subjectmicrosatellite marker
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpeanut
dc.subjectpest resistance
dc.subjectplant development
dc.subjectplant genetics
dc.subjectpolymorphic locus
dc.subjectspecies cultivation
dc.subjectArachis
dc.subjectArachis hypogaea
dc.titleTransferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachisen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/gmb/iaboutj.htm
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto Agronômico (IAC)
dc.description.affiliationUniversidade Estadual Paulista - UNESP Laboratório de Biotecnologia e Genética Molecular Departamento de Genética, Botucatu, SP
dc.description.affiliationInstituto Agronômico de Campinas, Caixa Postal 28, 13001-970 Campinas, SP
dc.description.affiliationUnespUniversidade Estadual Paulista - UNESP Laboratório de Biotecnologia e Genética Molecular Departamento de Genética, Botucatu, SP
dc.identifier.doi10.1590/S1415-47572006000300021
dc.identifier.scieloS1415-47572006000300021
dc.identifier.wosWOS:000240328500021
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-33748652069
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
dc.identifier.file2-s2.0-33748652069.pdf
dc.identifier.lattes6903215575686010
unesp.author.lattes6903215575686010
unesp.author.orcid0000-0002-6029-1925[2]
dc.relation.ispartofjcr1.493
dc.relation.ispartofsjr0,638
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