The use of planarians as in vivo animal model to study laser biomodulation effects
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Data
2007-04-30
Autores
Munin, Egberte
Garcia, Neila Maria Rocha
Braz, Allison Gustave
De Souza, Sandra Cristina
Alves, Leandro Procópio
Salgado, Miguel Angel Castillo [UNESP]
Pilla, Viviane
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Resumo
A variety of effects is attributed to the photo stimulation of tissues, such as improved healing of ulcers, analgesic and anti-inflammatory effects, stimulation of the proliferation of cells of different origins and stimulation of bone repair. Some investigations that make qualitative evaluations, like wound healing and evaluation of pain and edema, can be conducted in human subjects. However, deeper investigations on the mechanisms of action of the light stimulus and other quantitative works that requires biopsies or destructive analysis has to be carried out in animal models or in cell cultures. In this work, we propose the use of planarians as a model to study laser-tissue interaction. Contrasting with cell cultures and unicellular organisms, planarians are among the simplest organism having tissue layers, central nerve system, digestive and excretory system that might have been platforms for the evolution of the complex and highly organized tissues and organs found in higher organisms. For the present study, 685 nm laser radiation was employed. Planarians were cut transversally, in a plane posterior to the auricles. The body fragments were left to regenerate and the proliferation dynamics of stem cells was studied by using histological analysis. Maximum cell count was obtained for the laser treated group at the 4th experimental day. At that experimental time, we also had the largest difference between the irradiated and the non-irradiated control group. We concluded that the studied flatworm could be an interesting animal model for in vivo studies of laser-tissue interactions.
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Neoblast, Planarian, Regeneration, Stem cell, Biological organs, Cell culture, Computer simulation, Laser pulses, Orthopedics, Tissue engineering, Anti-inflammatory effects, Laser biomodulation effects, Living systems studies
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Progress in Biomedical Optics and Imaging - Proceedings of SPIE, v. 6435.