Estudo da reatividade cruzada em amostras de soro de cães positivos para Leishmania sp., Babesia canis e Ehrlichia canis, pelo ensaio imunoenzimático indireto e pela reação de imunofluorescência indireta
Alternative titleA study of cross-reactivity in serum samples from dogs positive for Leishmania sp., Babesia canis and Ehrlichia canis in enzyme-linked immunosorbent assay and indirect fluorescent antibody test.
MetadataShow full item record
To verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used in human visceral leishmaniasis control programs, serum samples from leishmaniasis endemic and non-endemic areas were collected and tested by Indirect Fluorescent Antibody (IFAT) and Enzyme-linked immunosorbent assay (ELISA). All serum samples from endemic areas were positive for Leishmania sp., by ELISA and IFAT, 51% positive for Babesia canis and 43% for Ehrlichia canis by IFAT. None of the serum samples from non-endemic areas were positive for Leishmania sp., by IFAT, but 67% were positive for B. canis and 78% for E. canis using the same test. When tested by ELISA for Leishmania sp., four samples from non-endemic area were positive. These dogs were then located and no clinical signs, parasites or antibody was detected in new tests for a six month period. Only one of these 4 samples was positive for B. canis by IFAT and ELISA and three for E. canis by IFAT. The results of the work suggest a co-infection in the endemic area and no serological cross-reaction among these parasites by IFAT and ELISA.
How to cite this document
Showing items related by title, author, creator and subject.
Toxoplasma gondii: Comparison of a rhoptry-ELISA with IFAT and MAT for antibody detection in sera of experimentally infected pigs Garcia, João Luis; Navarro, Italmar Teodorico; Vidotto, Odilon; Gennari, Solange Maria; Machado, Rosângela Zacarias ; Pereira, Ademir Benedito da Luz; Sinhorini, Idercio Luiz (Experimental Parasitology, 2006) [Artigo]Indirect ELISA and IFAT have been reported to be more sensitive and specific than agglutination tests. However, MAT is cheaper, easier than the others and does not need special equipment. The purpose of this study was to ...
Victor Canettieri, Antonio Carlos ; Kretchetoff, Fujiko Yamasiro; Koga Ito, Cristiane Yumi ; Moreira, Daniella ; Condino Fujarra, Fabio José; Unterkircher, Carmelinda Schmidt (Brazilian Oral Research, 2006) [Artigo]Several studies have been conducted in the last decades aiming to obtain an anti-canies vaccine, however some studies have demonstrated cross reactivity between Streptococcus mutans surface antigens and the human cardiac ...
Autoimmunity does not contribute to the highly prevalent glucose metabolism disturbances in a Japanese Brazilian population Hashimoto, Silvia M.; Hirai, A. T.; Kikuchi, M.; Cardoso, M. A. ; Tomita, N.; Chaim, R.; Wakisaka, K.; Gimeno, Suely G.A.; Matsumura, Luiza; Franco, Laercio J. et al. (Ethnicity and Disease, 2007) [Artigo]The Japanese Brazilian population has one of the highest prevalences of diabetes worldwide. Despite being non-obese according to standard definitions, their body fat distribution is typically central. We investigated whether ...