Comparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design
Data
2011-01-15
Autores
Giese, Ellen C. [UNESP]
Dekker, Robert F.H.
Scarminio, Ieda S.
Barbosa, Aneli M.
da Silva, Roberto [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Botryosphaeria rhodina MAMB-05 produced β-1,3-glucanases and botryosphaeran when grown on glucose, while Trichoderma harzianum Rifai only produced the enzyme. A comparison of long-term cultivation (300h) by B. rhodina demonstrated a correlation between the formation of botryosphaeran (48h) and its consumption (after 108h), and de-repression of β-1,3-glucanase synthesis when glucose was depleted from the nutrient medium, whereas for T. harzianum enzyme production commenced during exponential growth. Growth profiles and levels of β-1,3-glucanases produced by both fungi on botryosphaeran also differed, as well as the production of β-1,3-glucanases and β-1,6-glucanases on glucose, lactose, laminarin, botryosphaeran, lasiodiplodan, curdlan, Brewer's yeast powder and lyophilized fungal mycelium, which were dependent upon the carbon source used. A statistical mixture-design used to optimize β-1,3-glucanase production by both fungi evaluated botryosphaeran, glucose and lactose concentrations as variables. For B. rhodina, glucose and lactose promoted enzyme production at the same levels (2.30UmL -1), whereas botryosphaeran added to these substrates exerted a synergic effect favorable for β-glucanase production by T. harzianum (4.25UmL -1). © 2010 Elsevier B.V.
Descrição
Palavras-chave
β-1,3-Glucanases, Botryosphaeran, Statistical mixture-design optimization, Botryosphaeria rhodina MAMB-05, Carbon source, Curdlan, De-repression, Enzyme production, Exponential growth, Glucanase, Laminarins, Nutrient medium, Synergic effects, Trichoderma harzianum Rifai, Design, Enzymes, Fungi, Glucose, Mixtures, Optimization, 1,3 beta glucanase, curdlan, glucose, lactose, laminaran, botryosphaeria rhodina, comparative study, correlation analysis, enzyme substrate, enzyme synthesis, fungus, Hypocrea lixii, mycelium, nonhuman, priority journal, statistical analysis, Botryosphaeria rhodina
Como citar
Biochemical Engineering Journal, v. 53, n. 2, p. 239-243, 2011.