Prostatic stromal cells of old gerbils respond to steroidal blockades creating a microenvironment similar to reactive stroma
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Data
2011-04-01
Autores
Campos, Silvana G.P. [UNESP]
Gonalves, Bianca F.
Scarano, Wellerson R. [UNESP]
Ribeiro, Daniele Lisboa [UNESP]
Góes, Rejane M. [UNESP]
Taboga, Sebastião R. [UNESP]
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Resumo
The present study examined the changes in prostatic stroma of old gerbils (18 months) submitted to orchiectomy associated or not with steroidal blockades. Animals were divided into six groups, all surgically castrated except the control group composed of intact animals. The other two controls were formed by castrated animals, one that received and one that did not receive the drug vehicle. In the experimental groups, doses of flutamide (10 mg/kg/day) and/or tamoxifen (1 mg/kg/48 h) were applied for 1, 3, 7 and 30 days post-castration. The methodologies involved were: morphological (HE, Gömöri reticulin, Picrosirius-hematoxylin), immunohistochemical (tenascin, type IV collagen) and ultrastructural analyses. Gradually, the epithelial compartment was significantly exceeded in volume by the stromal compartment, characterizing regression but not atrophy of the gland. The smooth muscle cell frequency increased significantly after 30 days and participated effectively in the stromal increase. Large collagen I and tenascin deposits in the subepithelial region were a hallmark of prostatic acini in the experimental groups up to 7 days, while in the 30-day group these elements practically disappear. Fibroblasts with reactive aspect, changes in basement membrane structure and maintenance and/or increase of blood vessels were also associated with treatments. These results showed, in part, the sensitivity of stromal components to suppressed hormones and favored the creation of a differentiated glandular microenvironment. Therefore, the data suggest the importance of considering aging when analyzing aspects of prostatic regression between rodents and humans after hormonal ablation. © 2011 Elsevier Masson SAS. All rights reserved.
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Castration, Old gerbils, Prostate, collagen type 4, flutamide, tamoxifen, tenascin, aged, animal cell, animal experiment, animal tissue, castration, cell structure, controlled study, epithelium, fibroblast, gerbil, immunohistochemistry, male, nonhuman, priority journal, prostate, prostatic stromal cell, stroma cell, ultrastructure
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Biomedicine and Aging Pathology, v. 1, n. 2, p. 97-106, 2011.