Use of reverse transcriptase polymerase chain reaction (RT-PCR) in molecular screening of Newcastle disease virus in poultry and free-living bird populations
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Data
2012-09-17
Autores
de Oliveira Torres Carrasco, Adriano
Rodrigues, Juliana Nogueira Martins
Seki, Meire Christina [UNESP]
de Moraes, Fabricio Edgar [UNESP]
Silva, Jaqueline Raymondi [UNESP]
Durigon, Edison Luis
Pinto, Aramis Augusto [UNESP]
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Resumo
The aim of this study was to evaluate a simple molecular method of reverse transcriptase polymerase chain reaction (RT-PCR) to differentiate Newcastle disease virus strains according to their pathogenicity, in order to use it in molecular screening of Newcastle disease virus in poultry and free-living bird populations. Specific primers were developed to differentiate LaSota-LS-(vaccine strain) and Sao Joao do Meriti-SJM-strain (highly pathogenic strain). Chickens and pigeons were experimentally vaccinated/infected for an in vivo study to determine virus shedding in feces. Validation of sensitivity and specificity of the primers (SJM and LS) by experimental models used in the present study and results obtained in the molecular analysis of the primers by BLAST made it possible to generalize results. The development of primers that differentiate the level of pathogenicity of NDV stains is very important, mainly in countries where real-time RT-PCR is still not used as a routine test. These primers were able to determine the presence of the agent and to differentiate it according to its pathogenicity. © 2012 Springer Science+Business Media B.V.
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Diagnosis, Experimental infection, Newcastle disease virus, Poultry, Aves, Columba, Gallus gallus, primer DNA, animal, animal disease, bird disease, chicken, evaluation, feces, genetics, isolation and purification, methodology, Newcastle disease, Newcastle disease paramyxovirus, pigeons and doves, reverse transcription polymerase chain reaction, sensitivity and specificity, virology, virus shedding, Animals, Chickens, Columbidae, DNA Primers, Feces, Newcastle Disease, Poultry Diseases, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity, Virus Shedding
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Tropical Animal Health and Production, v. 45, n. 1, p. 569-576, 2012.