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dc.contributor.authorSerpeloni, Juliana Mara
dc.contributor.authorMazzaron Barcelos, Gustavo Rafael
dc.contributor.authorMori, Mateus Prates
dc.contributor.authorYanagui, Karina
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorVaranda, Eliana Aparecida [UNESP]
dc.contributor.authorde Syllos Colus, Ilce Mara
dc.date.accessioned2014-05-20T13:24:05Z
dc.date.available2014-05-20T13:24:05Z
dc.date.issued2011-07-01
dc.identifierhttp://dx.doi.org/10.1016/j.etp.2010.03.011
dc.identifier.citationExperimental and Toxicologic Pathology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 63, n. 5, p. 499-504, 2011.
dc.identifier.issn0940-2993
dc.identifier.urihttp://hdl.handle.net/11449/7395
dc.description.abstractThe Miconia genus, a plant widely used for medicine, occurs in tropical America and its extracts and isolated compounds have demonstrated antibiotic, antitumoral, analgesic and antimalarial activities. However, no study concerning its genotoxicity has been conducted and it is necessary to determine its potential mutagenic effects to develop products and chemicals from these extracts. This study assessed the cytotoxicity, mutagenicity and the protective effects of methanolic extracts from Miconia species on Chinese hamster lung fibroblast cell cultures (V79). The cytotoxicity was evaluated using a clonogenic assay. Cultures exposed to the extract of Miconia albicans up to a concentration of 30 mu g/mL, M. cabucu up to 40 mu g/mL, M. albicans up to 40 mu g/mL and M. stenostachya up to 60 mu g/mL exhibited a cytotoxic effect on the cells. The clonogenic assay used three non-cytotoxic concentrations (5, 10 and 20 mu g/mL) to evaluate mutagenicity and antimutagenicity of the extracts. Cultures were treated with these three extract concentrations (mutagenicity test) or the extract associated with doxorubicin (DXR) (antimutagenicity test) in three protocols (pre-, simultaneous and post-treatments). Distilled water and DXR were used as negative and positive controls, respectively. In the micronucleus (MN) test, a significant reduction was observed in MN frequency in cultures treated with DXR and extracts compared to those receiving only DXR; a significant reduction was also observed for the presence of mutagenicity in all treatments. This study confirmed the safe use of Miconia extracts at the concentrations tested and reinforced the therapeutic properties previously described for Miconia species by showing their protective effects on doxorubicin-induced mutagenicity. (C) 2010 Elsevier GmbH. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent499-504
dc.language.isoeng
dc.publisherElsevier Gmbh, Urban & Fischer Verlag
dc.relation.ispartofExperimental and Toxicologic Pathology
dc.sourceWeb of Science
dc.subjectMicronucleus testen
dc.subjectMiconiaen
dc.subjectCytotoxicityen
dc.subjectMutagenicityen
dc.subjectAntimutagenicityen
dc.subjectClonogenic assayen
dc.titleCytotoxic and mutagenic evaluation of extracts from plant species of the Miconia genus and their influence on doxorubicin-induced mutagenicity: An in vitro analysisen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier Gmbh, Urban & Fischer Verlag
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniv São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Bromatol & Toxicol, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniversidade Estadual de Londrina (UEL), Ctr Ciencias Biol, Dept Biol Geral, Londrina, PR, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Dept Quim Organ, Araraquara, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, Bauru, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Dept Quim Organ, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, Bauru, SP, Brazil
dc.identifier.doi10.1016/j.etp.2010.03.011
dc.identifier.wosWOS:000291706600013
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquarapt
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências, Baurupt
dc.identifier.orcid0000-0003-3032-2556
unesp.author.orcid0000-0003-3032-2556[5]
dc.relation.ispartofjcr2.023
dc.relation.ispartofsjr0,551
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