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dc.contributor.authorGuerreiro-Tanomaru, Juliane Maria [UNESP]
dc.contributor.authorDe Faria-Júnior, Norberto Batista [UNESP]
dc.contributor.authorDuarte, Marco Antônio Húngaro
dc.contributor.authorOrdinola-Zapata, Ronald
dc.contributor.authorGraeff, Márcia Sirlene Zardin
dc.contributor.authorTanomaru-Filho, Mário [UNESP]
dc.date.accessioned2014-05-27T11:28:34Z
dc.date.available2014-05-27T11:28:34Z
dc.date.issued2013-03-01
dc.identifierhttp://dx.doi.org/10.1016/j.joen.2012.09.027
dc.identifier.citationJournal of Endodontics, v. 39, n. 3, p. 346-350, 2013.
dc.identifier.issn0099-2399
dc.identifier.urihttp://hdl.handle.net/11449/74675
dc.description.abstractIntroduction: The aim of this study was to compare Enterococcus faecalis biofilm formation on different substrates. Methods: Cell culture plates containing growth medium and E. faecalis (ATCC 29212) were used to grow biofilm on bovine dentin, gutta-percha, hydroxyapatite, or bovine bone. Substrates were incubated at 37°C for 14 or 21 days, and the medium was changed every 48 hours. After the growth induction periods, specimens (n = 5 per group and per induction period) were stained by using Live/Dead, and the images were analyzed under a confocal microscope. The total biovolume (μm3), live bacteria biovolume (μm3), and substrate coverage (%) were quantified by using the BioImage-L software. Results obtained were analyzed by nonparametric tests (P =.05). Results: Biofilm formation was observed in all groups. Gutta-percha had the lowest total biovolume at 14 days (P <.05) and hydroxyapatite the highest at 21 days (P <.05). No significant difference was observed in green biovolume at 14 days. At 21 days, however, hydroxyapatite had the highest volume (P <.05). The percentages of coverage were similar among all substrates at 21 days (P >.05), but at 14 days, bovine bone presented the highest coverage (P <.05). Conclusions: E. faecalis was capable of forming biofilm on all substrates during both growth periods; hydroxyapatite presented the highest rates of biofilm formation. The type of substrate influenced the biofilm characteristics, according to the parameters evaluated. © 2013 American Association of Endodontists.en
dc.format.extent346-350
dc.language.isoeng
dc.relation.ispartofJournal of Endodontics
dc.sourceScopus
dc.subjectBiofilm
dc.subjectconfocal microscopy
dc.subjectEnterococcus faecalis
dc.titleComparative analysis of Enterococcus faecalis biofilm formation on different substratesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationDepartment of Restorative Dentistry Araraquara Dental School UNESP-Univ Estadual Paulista, Rua Humaitá, 1680, 14801-903 Araraquara, SP
dc.description.affiliationDepartment of Restorative Dentistry Bauru Dental School USP-University of São Paulo, Bauru, São Paulo
dc.description.affiliationIntegrated Research Center Bauru Dental School USP-University of São Paulo, Bauru, São Paulo
dc.description.affiliationUnespDepartment of Restorative Dentistry Araraquara Dental School UNESP-Univ Estadual Paulista, Rua Humaitá, 1680, 14801-903 Araraquara, SP
dc.identifier.doi10.1016/j.joen.2012.09.027
dc.identifier.wosWOS:000316031200006
dc.rights.accessRightsAcesso restrito
dc.identifier.scopus2-s2.0-84873719314
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
dc.relation.ispartofjcr2.886
dc.relation.ispartofsjr1,585
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