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dc.contributor.authorCosta, Nadia Lago
dc.contributor.authorValadares, Marize Campos
dc.contributor.authorSouza, Pedro Paulo Chaves [UNESP]
dc.contributor.authorMendonça, Elismauro Francisco
dc.contributor.authorOliveira, José Carlos
dc.contributor.authorSilva, Tarcíla Aparecida
dc.contributor.authorBatista, Aline Carvalho
dc.date.accessioned2014-05-27T11:28:34Z
dc.date.available2014-05-27T11:28:34Z
dc.date.issued2013-03-01
dc.identifierhttp://dx.doi.org/10.1016/j.oraloncology.2012.09.012
dc.identifier.citationOral Oncology, v. 49, n. 3, p. 216-223, 2013.
dc.identifier.issn1368-8375
dc.identifier.issn1879-0593
dc.identifier.urihttp://hdl.handle.net/11449/74683
dc.description.abstractObjective: To evaluate and characterize macrophage populations (M1/M2) in the tumor microenvironment of oral cavity squamous cell carcinoma (OCSCC). The relationship between macrophages and clinicopathological factors, such as survival data, lymph node metastasis, tumoral proliferation, and WHO histological grading are also analyzed. Materials and methods: The samples consisted of surgically excised specimens from patients with non-metastatic and metastatic OCSCC and normal oral mucosa (control). Immunohistochemistry, flow cytometry, and qRT-PCR were used to evaluate macrophage populations and the expression of pro- (IL-12, IL-23, and INF-γ) and anti-inflammatory (IL-10 and TGF-β) cytokines. The level required for statistical significance was defined as p < 0.05. Results: The data showed a predominance of M2 phenotype (high percentage of IL-10+TGF-β+) macrophages in the tumor microenvironment of OCSCC. A higher percentage of macrophages expressing TGF-β was seen in the OCSCC group when compared with healthy individuals. The assessment of mRNA expression also presented a greater expression of anti-inflammatory cytokines TGFβ and IL10 in OCSCC when compared with the control group. The percentage of macrophages, demonstrated by immunohistochemistry, was significantly higher in the metastatic OCSCC group than in the non-metastatic and control groups. The log-rank test also showed that the mean survival time for patients with high levels of macrophages was less (44 months) when compared with patients with a low percentage of such cells (93 months). Conclusion: A predominance of the M2 phenotype in the tumor microenvironment of OCSCC could contribute to local immunosuppression, via TGF-β production, and consequently greater lymph node involvement and reduced patient survival time. © 2012 Elsevier Ltd. All rights reserved.en
dc.format.extent216-223
dc.language.isoeng
dc.relation.ispartofOral Oncology
dc.sourceScopus
dc.subjectCytokines
dc.subjectInterferon-gamma
dc.subjectInterleukin-10
dc.subjectInterleukin-12
dc.subjectInterleukin-23
dc.subjectMacrophages
dc.subjectOral cancer
dc.subjectSquamous cell carcinoma
dc.subjectTransforming growth factor beta
dc.subjectgamma interferon
dc.subjectinterleukin 10
dc.subjectinterleukin 12
dc.subjectinterleukin 23
dc.subjectmessenger RNA
dc.subjecttransforming growth factor beta
dc.subjectadult
dc.subjectaged
dc.subjectcancer invasion
dc.subjectcancer staging
dc.subjectcancer survival
dc.subjectcell population
dc.subjectcell proliferation
dc.subjectcontrolled study
dc.subjectcytokine release
dc.subjectfemale
dc.subjectflow cytometry
dc.subjecthistopathology
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectlymph node metastasis
dc.subjectmacrophage
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmouth mucosa
dc.subjectmouth squamous cell carcinoma
dc.subjectnucleotide sequence
dc.subjectphenotype
dc.subjectpriority journal
dc.subjectretrospective study
dc.subjectreverse transcription polymerase chain reaction
dc.subjectsurvival time
dc.subjecttumor associated leukocyte
dc.subjecttumor localization
dc.subjecttumor microenvironment
dc.subjecttumor volume
dc.subjectAdult
dc.subjectAged
dc.subjectAged, 80 and over
dc.subjectAntigens, CD11
dc.subjectCarcinoma, Squamous Cell
dc.subjectCell Count
dc.subjectCell Proliferation
dc.subjectFemale
dc.subjectFollow-Up Studies
dc.subjectHumans
dc.subjectImmune Tolerance
dc.subjectInflammation Mediators
dc.subjectLymphatic Metastasis
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMouth Neoplasms
dc.subjectNeoplasm Grading
dc.subjectNeoplasm Invasiveness
dc.subjectRetrospective Studies
dc.subjectSurvival Rate
dc.subjectTransforming Growth Factor beta
dc.subjectTumor Microenvironment
dc.titleTumor-associated macrophages and the profile of inflammatory cytokines in oral squamous cell carcinomaen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionGoiás Combat Cancer Association
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.description.affiliationDepartment of Stomatology (Oral Pathology) Dental School Federal University of Goiás, Goiânia
dc.description.affiliationLaboratory of Cellular Pharmacology and Toxicology Faculty of Pharmacy Federal University of Goiás, Goiânia
dc.description.affiliationDepartment of Physiology and Pathology Araraquara School of Dentistry Sao Paulo State University, Araraquara, Sao Paulo
dc.description.affiliationDivision of Head and Neck Araújo Jorge Hospital Goiás Combat Cancer Association, Goiânia
dc.description.affiliationDepartment of Oral Surgery and Pathology Dental School Federal University of Minas Gerais, Belo Horizonte
dc.description.affiliationUnespDepartment of Physiology and Pathology Araraquara School of Dentistry Sao Paulo State University, Araraquara, Sao Paulo
dc.identifier.doi10.1016/j.oraloncology.2012.09.012
dc.identifier.wosWOS:000314871900011
dc.rights.accessRightsAcesso restrito
dc.identifier.scopus2-s2.0-84873412294
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
dc.relation.ispartofjcr4.636
dc.relation.ispartofsjr1,912
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