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dc.contributor.authorScorzoni, Liliana [UNESP]
dc.contributor.authorde Lucas, Maria Pilar
dc.contributor.authorMesa-Arango, Ana Cecilia
dc.contributor.authorFusco-Almeida, Ana Marisa [UNESP]
dc.contributor.authorLozano, Encarnación
dc.contributor.authorCuenca-Estrella, Manuel
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.authorZaragoza, Oscar
dc.date.accessioned2014-05-27T11:28:44Z
dc.date.available2014-05-27T11:28:44Z
dc.date.issued2013-03-28
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0060047
dc.identifier.citationPLoS ONE, v. 8, n. 3, 2013.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11449/74891
dc.description.abstractThe incidence of opportunistic fungal infections has increased in recent decades due to the growing proportion of immunocompromised patients in our society. Candida krusei has been described as a causative agent of disseminated fungal infections in susceptible patients. Although its prevalence remains low among yeast infections (2-5%), its intrinsic resistance to fluconazole makes this yeast important from epidemiologic aspects. Non mammalian organisms are feasible models to study fungal virulence and drug efficacy. In this work we have used the lepidopteran Galleria mellonella and the nematode Caenorhabditis elegans as models to assess antifungal efficacy during infection by C. krusei. This yeast killed G. mellonella at 25, 30 and 37°C and reduced haemocytic density. Infected larvae melanized in a dose-dependent manner. Fluconazole did not protect against C. krusei infection, in contrast to amphotericin B, voriconazole or caspofungin. However, the doses of these antifungals required to obtain larvae protection were always higher during C. krusei infection than during C. albicans infection. Similar results were found in the model host C. elegans. Our work demonstrates that non mammalian models are useful tools to investigate in vivo antifungal efficacy and virulence of C. krusei. © 2013 Scorzoni et al.en
dc.language.isoeng
dc.relation.ispartofPLOS ONE
dc.sourceScopus
dc.subjectamphotericin B
dc.subjectcaspofungin
dc.subjectfluconazole
dc.subjectvoriconazole
dc.subjectanimal tissue
dc.subjectantifungal activity
dc.subjectantifungal susceptibility
dc.subjectarthropod larva
dc.subjectCaenorhabditis elegans
dc.subjectCandida krusei
dc.subjectcandidiasis
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectcytolysis
dc.subjectdrug efficacy
dc.subjectfungal virulence
dc.subjectGalleria mellonella
dc.subjecthistopathology
dc.subjectin vitro study
dc.subjectminimum inhibitory concentration
dc.subjectnonhuman
dc.subjectphagocytosis
dc.subjectprotection
dc.titleAntifungal Efficacy during Candida krusei Infection in Non-Conventional Models Correlates with the Yeast In Vitro Susceptibility Profileen
dc.typeArtigo
dcterms.licensehttp://www.plos.org/open-access/
dc.contributor.institutionInstituto de Salud Carlos III
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Antioquia
dc.description.affiliationMycology Reference Laboratory National Centre for Microbiology Instituto de Salud Carlos III, Madrid
dc.description.affiliationLaboratório de Micologia Clínica Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista de São Paulo, Araraquara
dc.description.affiliationDepartment of Cellular Biology National Centre for Microbiology Instituto de Salud Carlos III, Madrid
dc.description.affiliationGroup of Investigative Dermatology University of Antioquia, Medellín
dc.description.affiliationUnespLaboratório de Micologia Clínica Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista de São Paulo, Araraquara
dc.identifier.doi10.1371/journal.pone.0060047
dc.identifier.wosWOS:000317262200072
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-84875543551
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
dc.identifier.file2-s2.0-84875543551.pdf
dc.identifier.orcid0000-0002-8059-0826
unesp.author.lattes3716273524139678[4]
unesp.author.orcid0000-0002-8059-0826[7]
unesp.author.orcid0000-0002-2115-8988[4]
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
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