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dc.contributor.authorDa Silva, Bruno Rocha
dc.contributor.authorDe Freitas, Victor Aragão Abreu
dc.contributor.authorCarneiro, Victor Alves
dc.contributor.authorArruda, Francisco Vassiliepe Sousa
dc.contributor.authorLorenzón, Esteban Nicolás [UNESP]
dc.contributor.authorDe Aguiar, Andréa Silvia Walter
dc.contributor.authorCilli, Eduardo Maffud [UNESP]
dc.contributor.authorCavada, Benildo Sousa
dc.contributor.authorTeixeira, Edson Holanda
dc.date.accessioned2014-05-27T11:28:45Z
dc.date.available2014-05-27T11:28:45Z
dc.date.issued2013-04-01
dc.identifierhttp://dx.doi.org/10.1016/j.peptides.2012.12.001
dc.identifier.citationPeptides, v. 42, p. 78-83.
dc.identifier.issn0196-9781
dc.identifier.issn1873-5169
dc.identifier.urihttp://hdl.handle.net/11449/74938
dc.description.abstractThe peptide LYS-[TRP6]-Hy-A1 (Lys-a1) is a synthetic derivative of the peptide Hy-A1, initially isolated from the frog species Hypsiboas albopunctatus. According to previous research, it is a molecule with broad antimicrobial activity. The objective of this study was to evaluate the antimicrobial activity of the synthetic peptide Lys-a1 (KIFGAIWPLALGALKNLIK- NH2) on the planktonic and biofilm growth of oral bacteria. The methods used to evaluate antimicrobial activity include the following: determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in microtiter plates for growth in suspension and quantification of biomass by crystal violet staining and counting of colony forming units for biofilm growth. The microorganisms Streptococcus oralis, Streptococcus sanguinis, Streptococcus parasanguinis, Streptococcus salivarius, Streptococcus mutans and Streptococcus sobrinus were grown in Brain Heart Infusion broth at 37 °C under atmospheric pressure with 10% CO2. The peptide was solubilized in 0.1% acetic acid (v/v) at various concentrations (500-1.9 μg mL-1). Chlorhexidine gluconate 0.12% was used as the positive control, and BHI culture medium was used as the negative control. The tested peptide demonstrated a remarkable antimicrobial effect, inhibiting the planktonic and biofilm growth of all strains tested, even at low concentrations. Thus, the peptide Lys-a1 is an important source for potential antimicrobial agents, especially for the control and prevention of microbial biofilms, which is one of the most important factors in cariogenic processes. © 2012 Elsevier Inc.en
dc.format.extent78-83
dc.language.isoeng
dc.relation.ispartofPeptides
dc.sourceScopus
dc.subjectAntimicrobial peptide
dc.subjectBiofilms
dc.subjectDental caries
dc.subjectStreptococcus mutans
dc.subjectacetic acid
dc.subjectchlorhexidine gluconate
dc.subjectcrystal violet
dc.subjectpolypeptide antibiotic agent
dc.subjectsynthetic peptide Lys a1
dc.subjectunclassified drug
dc.subjectantibacterial activity
dc.subjectatmospheric pressure
dc.subjectbacterial growth
dc.subjectbacterium colony
dc.subjectbiofilm
dc.subjectcontrolled study
dc.subjectdrug solubility
dc.subjectgrowth inhibition
dc.subjectmicrotiter plate assay
dc.subjectminimum bactericidal concentration
dc.subjectminimum inhibitory concentration
dc.subjectnonhuman
dc.subjectplankton
dc.subjectpriority journal
dc.subjectStreptococcus
dc.subjectStreptococcus oralis
dc.subjectStreptococcus parasanguinis
dc.subjectStreptococcus salivarius
dc.subjectStreptococcus sanguinis
dc.subjectStreptococcus sobrinus
dc.subjectAnura
dc.subjectBacteria (microorganisms)
dc.titleAntimicrobial activity of the synthetic peptide Lys-a1 against oral streptococcien
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionFederal University of Ceara
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFederal University of Ceará
dc.description.affiliationBioMol Group/DPML/LIBS Integrate Biomolecules Laboratory Federal University of Ceara, Fortaleza, CE
dc.description.affiliationInstitute of Chemistry Universidade Estadual Paulista-UNESP, Araraquara, SP
dc.description.affiliationClinical Dentistry Department Federal University of Ceara, Fortaleza, CE
dc.description.affiliationBioMol Group/DBBM/BioMol-Lab Federal University of Ceará, Fortaleza, CE
dc.description.affiliationUnespInstitute of Chemistry Universidade Estadual Paulista-UNESP, Araraquara, SP
dc.identifier.doi10.1016/j.peptides.2012.12.001
dc.identifier.wosWOS:000320492800011
dc.rights.accessRightsAcesso restrito
dc.identifier.scopus2-s2.0-84874528992
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
dc.identifier.lattes9424346762460416
dc.identifier.orcid0000-0002-4767-0904
unesp.author.lattes9424346762460416
unesp.author.orcid0000-0002-4767-0904[7]
dc.relation.ispartofjcr2.851
dc.relation.ispartofsjr1,001
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