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dc.contributor.authorCristofalo, R.
dc.contributor.authorBannwart-Castro, C. F.
dc.contributor.authorMagalhães, C. G.
dc.contributor.authorBorges, V. T.
dc.contributor.authorPeraçoli, José Carlos [UNESP]
dc.contributor.authorWitkin, S. S.
dc.contributor.authorPeraçoli, M. T.
dc.date.accessioned2014-05-27T11:28:48Z
dc.date.available2014-05-27T11:28:48Z
dc.date.issued2013-04-01
dc.identifierhttp://dx.doi.org/10.3109/10715762.2013.765951
dc.identifier.citationFree radical research, v. 47, n. 4, p. 268-275, 2013.
dc.identifier.issn1029-2470
dc.identifier.urihttp://hdl.handle.net/11449/75026
dc.description.abstractSilibinin is a polyphenolic plant flavonoid with anti-inflammatory properties. The present study investigated the effect of silibinin on oxidative metabolism and cytokine production - tumor necrosis factor-alpha (TNF-α), interleukin (IL)12, granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-6, IL-10, and transforming growth factor beta (TGF-β1) - by peripheral blood monocytes (PBM) from preeclamptic pregnant women. It is a case-controlled study involving women with preeclampsia (PE, n = 30) compared with normotensive pregnant (NT, n = 30) and with non-pregnant (NP, n = 30) women. Monocytes were obtained and cultured with or without silibinin (5 μM or 50 μM) for 18 h. Superoxide anion (O2-) and hydrogen peroxide (H2O2) release were determined by specific assays, and cytokine levels were determined by immunoenzymatic assays (ELISA). Monocytes from preeclamptic women cultured without stimulus released higher levels of O22, H2O2 and TNF-α, and lower levels of IL-10 and TGF-β1 than did monocytes from NT and NP women. Treatment in vitro with silibinin significantly inhibited spontaneous O2- and H2O2 release and TNF-α production by monocytes from preeclamptic women. The main effect of silibinin was obtained at 50 μM concentration. Thus, silibinin exerts anti-oxidative and anti-inflammatory effects on monocytes from preeclamptic pregnant women by inhibiting the in vitro endogenous release of reactive oxygen species and TNF-α production.en
dc.format.extent268-275
dc.language.isoeng
dc.relation.ispartofFree Radical Research
dc.sourceScopus
dc.subjectantiinflammatory agent
dc.subjectantioxidant
dc.subjectcytokine
dc.subjecthydrogen peroxide
dc.subjectinterleukin 10
dc.subjectinterleukin 6
dc.subjectsilibinin
dc.subjectsilymarin
dc.subjectsuperoxide
dc.subjecttransforming growth factor beta1
dc.subjecttumor necrosis factor alpha
dc.subjectblood
dc.subjectcell culture
dc.subjectcytology
dc.subjectdrug effect
dc.subjectfemale
dc.subjecthuman
dc.subjectmetabolism
dc.subjectmonocyte
dc.subjectoxidative stress
dc.subjectpreeclampsia
dc.subjectpregnancy
dc.subjectsignal transduction
dc.subjectAnti-Inflammatory Agents
dc.subjectAntioxidants
dc.subjectCells, Cultured
dc.subjectCytokines
dc.subjectFemale
dc.subjectHumans
dc.subjectHydrogen Peroxide
dc.subjectInterleukin-10
dc.subjectInterleukin-6
dc.subjectMonocytes
dc.subjectOxidative Stress
dc.subjectPre-Eclampsia
dc.subjectPregnancy
dc.subjectSignal Transduction
dc.subjectSilymarin
dc.subjectSuperoxides
dc.subjectTransforming Growth Factor beta1
dc.subjectTumor Necrosis Factor-alpha
dc.titleSilibinin attenuates oxidative metabolism and cytokine production by monocytes from preeclamptic women.en
dc.typeArtigo
dcterms.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.identifier.doi10.3109/10715762.2013.765951
dc.identifier.wosWOS:000316083100003
dc.rights.accessRightsAcesso restrito
dc.identifier.scopus2-s2.0-84881033336
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.author.orcid0000-0002-0936-9512[7]
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