Show simple item record

dc.contributor.authorLeite, Bruna
dc.contributor.authorGomes, Fernanda
dc.contributor.authorTeixeira, Pilar
dc.contributor.authorSouza, Clovis
dc.contributor.authorPizzolitto, Elisabeth
dc.contributor.authorOliveira, Rosário
dc.date.accessioned2014-05-27T11:29:01Z
dc.date.available2014-05-27T11:29:01Z
dc.date.issued2013-05-01
dc.identifierhttp://dx.doi.org/10.1016/j.eimc.2012.11.011
dc.identifier.citationEnfermedades Infecciosas y Microbiologia Clinica.
dc.identifier.issn0213-005X
dc.identifier.issn1578-1852
dc.identifier.urihttp://hdl.handle.net/11449/75208
dc.description.abstractIntroduction: Staphylococcus epidermidis is an organism commonly associated with infections caused by biofilms. Biofilms are less sensible to antibiotics and therefore are more difficult to eradicate. Linezolid and N-acetylcysteine (NAC), have demonstrated to be active against gram-positive microorganisms. Therefore and since linezolid and NAC have different modes of action, the main objective of this work was to investigate the single and synergistic effect of linezolid and NAC against S. epidermidis biofilms. Methods: This work reports the in vitro effect of linezolid and NAC against S. epidermidis biofilms, treated with MIC (4 mg ml-1) and 10×MIC of NAC, and MIC (1 μg ml-1) and peak serum concentration (PS = 18 μg ml-1) of linezolid alone and in combination. After exposure of S. epidermidis biofilms to linezolid and/or NAC for 24 h, several biofilm parameters were evaluated, namely the number of cultivable cells [colony forming unit (CFU) enumeration], total biofilm biomass and cellular activity. Results: When tested alone, NAC at 10×MIC was the most effective agent against S. epidermidis biofilms. However, the combination linezolid (MIC) + NAC (10×MIC) showed a synergistic effect and was the most biocidal treatment tested, promoting a 5 log reduction in the number of biofilm viable cells. Conclusion: This combination seems to be a potential candidate to combat infections caused by S. epidermidis biofilms, namely as a catheter lock solution therapy. © 2012 Elsevier España, S.L. All rights reserved.en
dc.language.isoeng
dc.relation.ispartofEnfermedades Infecciosas y Microbiologia Clinica
dc.sourceScopus
dc.subjectAntimicrobial agents
dc.subjectBiofilm
dc.subjectLinezolid
dc.subjectN-acetylcysteine
dc.subjectStaphylococcus epidermidis
dc.titleCombined effect of linezolid and N-acetylcysteine against Staphylococcus epidermidis biofilmsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.identifier.doi10.1016/j.eimc.2012.11.011
dc.identifier.wosWOS:000328810700005
dc.rights.accessRightsAcesso restrito
dc.identifier.scopus2-s2.0-84876719717
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.author.orcid0000-0003-0318-4733[2]
unesp.author.orcid0000-0003-4335-1460[4]
unesp.author.orcid0000-0003-2735-6608[3]
dc.relation.ispartofjcr1.707
dc.relation.ispartofsjr0,373
Localize o texto completo

Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record