Biostimulatory effects of low-level laser therapy on epithelial cells and gingival fibroblasts treated with zoledronic acid
Nenhuma Miniatura disponível
Data
2013-05-01
Autores
Basso, F. G.
Pansani, T. N. [UNESP]
Turrioni, A. P S [UNESP]
Kurachi, C.
Bagnato, V. S.
Hebling, J. [UNESP]
de Souza Costa, C. A. [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Low-level laser therapy (LLLT) has been considered as an adjuvant treatment for bisphosphonate-related osteonecrosis, presenting positive clinical outcomes. However, there are no data regarding the effect of LLLT on oral tissue cells exposed to bisphosphonates. This study aimed to evaluate the effects of LLLT on epithelial cells and gingival fibroblasts exposed to a nitrogen-containing bisphosphonate - zoledronic acid (ZA). Cells were seeded in wells of 24-well plates, incubated for 48 h and then exposed to ZA at 5 μM for an additional 48 h. LLLT was performed with a diode laser prototype - LaserTABLE (InGaAsP - 780 nm ± 3 nm, 25 mW), at selected energy doses of 0.5, 1.5, 3, 5, and 7 J cm-2 in three irradiation sessions, every 24 h. Cell metabolism, total protein production, gene expression of vascular endothelial growth factor (VEGF) and collagen type I (Col-I), and cell morphology were evaluated 24 h after the last irradiation. Data were statistically analyzed by Kruskal-Wallis and Mann-Whitney tests at 5% significance. Selected LLLT parameters increased the functions of epithelial cells and gingival fibroblasts treated with ZA. Gene expression of VEGF and Col-I was also increased. Specific parameters of LLLT biostimulated fibroblasts and epithelial cells treated with ZA. Analysis of these in vitro data may explain the positive in vivo effects of LLLT applied to osteonecrosis lesions. © 2013 Astro Ltd.
Descrição
Palavras-chave
Adjuvant treatment, Cell metabolism, Clinical outcome, Epithelial cells, Gingival fibroblasts, Low level laser therapy, Vascular endothelial growth factor, Zoledronic acid, Fibroblasts, Gene expression, Irradiation, Laser tissue interaction, Tissue, Cell culture
Como citar
Laser Physics, v. 23, n. 5, 2013.