Improved laboratory safety by decontamination of unstained sputum smears for acid-fast microscopy
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Data
2005-08-01
Autores
Giacomelli, LRB
Helbel, C.
Ogassawara, RLN
Barreto, AMW
Martins, F. M.
Cardoso, C. L.
Leite, CQF
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Amer Soc Microbiology
Resumo
Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. The smears were decontaminated by the tube and slide techniques. Phenol at 5%, glutaraldehyde at 2%, and buffered formalin at 3.7% for 1 min (tube technique) or for 10 min (slide technique) were effective in decontaminating sputum smears and preserved cell morphology and quantitative acid-fast microscopy results.
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Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 8, p. 4245-4248, 2005.