Serotonergic neurons in the nucleus raphé obscurus are not involved in the ventilatory and thermoregulatory responses to hypoxia in adult rats

Nenhuma Miniatura disponível

Data

2013-06-15

Autores

Da Silva, Glauber S.F.
Giusti, Humberto
Castro, Olagide W.
Garcia-Cairasco, Norberto
Gargaglioni, Luciane H. [UNESP]
Branco, Luiz G.S.
Glass, Mogens L.

Título da Revista

ISSN da Revista

Título de Volume

Editor

Resumo

The medullary raphé is an important component of the central respiratory network, playing a key role in CO2 central chemoreception. However, its participation in hypoxic ventilatory responses is less understood. In the present study, we assessed the role of nucleus raphé obscurus (ROb), and specifically 5-HT neurons confined in the ROb, on ventilatory and thermoregulatory responses to hypoxia. Chemical lesions of the ROb were performed using either ibotenic acid (non-specific lesion; control animals received PBS) or anti-SERT-SAP (5-HT specific lesion; control animals received IgG-SAP). Ventilation (VE; whole body plethysmograph) and body temperature (Tb; data loggers) were measured during normoxia (21% O2, N2 balance) and hypoxia exposure (7% O2, N2 balance, 1h) in conscious adult rats. Ibotenic acid or anti-SERT-SAP-induced lesions did not affect baseline values of VE and Tb. Similarly, both lesion procedures did not alter the ventilatory or thermoregulatory responses to hypoxia. Although evidence in the literature suggests a role of the rostral medullary raphé in hypoxic ventilatory responses, under the present experimental conditions our data indicate that caudal medullary raphé (ROb) and its 5-HT neurons neither participate in the tonic maintenance of breathing nor in the ventilatory and thermal responses to hypoxia. © 2013 Elsevier B.V.

Descrição

Palavras-chave

Body temperature, Caudal medullary raphé, Hypoxia, Pulmonary ventilation, Serotonin, ibotenic acid, animal experiment, animal tissue, blood gas analysis, body temperature, controlled study, hypoxia, immunohistochemistry, lung ventilation, male, nerve cell lesion, nonhuman, nucleus raphe obscurus, priority journal, rat, serotoninergic nerve cell, stereotaxic surgery, thermoregulation, tissue culture

Como citar

Respiratory Physiology and Neurobiology, v. 187, n. 2, p. 139-148, 2013.