Peripheral kappa and delta opioid receptors are involved in the antinociceptive effect of crotalphine in a rat model of cancer pain
Nenhuma Miniatura disponível
Data
2013-06-17
Autores
Brigatte, Patricia [UNESP]
Konno, Katsuhiro
Gutierrez, Vanessa Pacciari
Sampaio, Sandra Coccuzzo
Zambelli, Vanessa Olzon
Picolo, Gisele
Curi, Rui
Cury, Yara
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Cancer pain is an important clinical problem and may not respond satisfactorily to the current analgesic therapy. We have characterized a novel and potent analgesic peptide, crotalphine, from the venom of the South American rattlesnake Crotalus durissus terrificus. In the present work, the antinociceptive effect of crotalphine was evaluated in a rat model of cancer pain induced by intraplantar injection of Walker 256 carcinoma cells. Intraplantar injection of tumor cells caused the development of hyperalgesia and allodynia, detected on day 5 after tumor cell inoculation. Crotalphine (6 μg/kg), administered p.o., blocked both phenomena. The antinociceptive effect was detected 1 h after treatment and lasted for up to 48 h. Intraplantar injection of nor-binaltorphimine (50 g/paw), a selective antagonist of κ-opioid receptors, antagonized the antinociceptive effect of the peptide, whereas N,N-diallyl-Tyr-Aib-Phe-Leu (ICI 174,864, 10 μg/paw), a selective antagonist of δ-opioid receptors, partially reversed this effect. On the other hand, D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr amide (CTOP, 20 g/paw), an antagonist of μ-opioid receptors, did not modify crotalphine-induced antinociception. These data indicate that crotalphine induces a potent and long lasting opioid-mediated antinociception in cancer pain. © 2013 Elsevier Inc.
Descrição
Palavras-chave
Antinociception, Cancer pain, Crotalphine, Opioid receptors, Walker 256 carcinoma cells, antinociceptive agent, crotalphine, delta opiate receptor, kappa opiate receptor, morphine, unclassified drug, animal experiment, animal model, antinociception, cancer growth, cancer pain, drug effect, drug efficacy, locomotion, male, nonhuman, pain threshold, priority journal, rat
Como citar
Pharmacology Biochemistry and Behavior, v. 109, p. 1-7.