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dc.contributor.authorBertanha, Matheus [UNESP]
dc.contributor.authorMoroz, Andrei[UNESP]
dc.contributor.authorAlmeida, Rodrigo
dc.contributor.authorAlves, Flavia Cilene
dc.contributor.authorAcorci Valério, Michele Janegitz
dc.contributor.authorMoura, Regina [UNESP]
dc.contributor.authorDomingues, Maria Aparecida Custódio [UNESP]
dc.contributor.authorSobreira, Marcone Lima [UNESP]
dc.contributor.authorDeffune, Elenice [UNESP]
dc.date.accessioned2014-05-27T11:29:55Z
dc.date.available2014-05-27T11:29:55Z
dc.date.issued2013-07-03
dc.identifierhttp://dx.doi.org/10.1016/j.jvs.2013.05.032
dc.identifier.citationJournal of Vascular Surgery.
dc.identifier.issn0741-5214
dc.identifier.issn1097-6809
dc.identifier.urihttp://hdl.handle.net/11449/75907
dc.description.abstractBackground: Cardiovascular diseases remain leaders as the major causes of mortality in Western society. Restoration of the circulation through construction of bypass surgical treatment is regarded as the gold standard treatment of peripheral vascular diseases, and grafts are necessary for this purpose. The great saphenous vein is often not available and synthetic grafts have their limitations. Therefore, new techniques to produce alternative grafts have been developed and, in this sense, tissue engineering is a promising alternative to provide biocompatible grafts. This study objective was to reconstruct the endothelium layer of decellularized vein scaffolds, using mesenchymal stem cells (MSCs) and growth factors obtained from platelets. Methods: Fifteen nonpregnant female adult rabbits were used for all experiments. Adipose tissue and vena cava were obtained and subjected to MSCs isolation and tissue decellularization, respectively. MSCs were subjected to differentiation using endothelial inductor growth factor (EIGF) obtained from human platelet lysates. Immunofluorescence, histological and immunohistochemical analyses were employed for the final characterization of the obtained blood vessel substitute. Results: The scaffolds were successfully decellularized with sodium dodecyl sulfate. MSCs actively adhered at the scaffolds, and through stimulation with EIGF were differentiated into functional endothelial cells, secreting significantly higher quantities of von Willebrand factor (0.85 μg/mL; P < .05) than cells cultivated under the same conditions, without EIGF (0.085 μg/mL). Cells with evident morphologic characteristics of endothelium were seen at the lumen of the scaffolds. These cells also stained positive for fascin protein, which is highly expressed by differentiated endothelial cells. Conclusions: Taken together, the use of decellularized bioscaffold and subcutaneous MSCs seems to be a potential approach to obtain bioengineered blood vessels, in the presence of EIGF supplementation. © 2013 Society for Vascular Surgery.en
dc.language.isoeng
dc.relation.ispartofJournal of Vascular Surgery
dc.sourceScopus
dc.titleTissue-engineered blood vessel substitute by reconstruction of endothelium using mesenchymal stem cells induced by platelet growth factorsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.identifier.doi10.1016/j.jvs.2013.05.032
dc.identifier.wosWOS:000336363800031
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-84879483963
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
dc.identifier.file2-s2.0-84879483963.pdf
dc.identifier.lattes9646764071339214
dc.identifier.lattes9609324832591382
dc.identifier.lattes0585723113037140
dc.identifier.lattes4513014379461383
unesp.author.lattes9646764071339214
unesp.author.lattes9609324832591382
unesp.author.lattes0585723113037140
unesp.author.lattes6926124203948011[2]
unesp.advisor.lattes4513014379461383
unesp.author.orcid0000-0003-2271-5878[7]
unesp.author.orcid0000-0002-4498-9784[2]
unesp.author.orcid0000-0002-0533-3248[9]
unesp.author.orcid0000-0002-6851-2841[1]
dc.relation.ispartofjcr3.294
dc.relation.ispartofsjr2,308
dc.relation.ispartofsjr2,308
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