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dc.contributor.authorCamargo, Mariana S. [UNESP]
dc.contributor.authorPrieto, Aline M. [UNESP]
dc.contributor.authorResende, Flavia A. [UNESP]
dc.contributor.authorBoldrin, Paula K. [UNESP]
dc.contributor.authorCardoso, Cassia R.P. [UNESP]
dc.contributor.authorFernández, Mariana F.
dc.contributor.authorMolina-Molina, José Manuel
dc.contributor.authorOlea, Nicolás
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorCuesta-Rubio, Osmany
dc.contributor.authorVaranda, Eliana Aparecida [UNESP]
dc.date.accessioned2014-05-27T11:30:04Z
dc.date.available2014-05-27T11:30:04Z
dc.date.issued2013-07-31
dc.identifierhttp://dx.doi.org/10.1186/1472-6882-13-201
dc.identifier.citationBMC Complementary and Alternative Medicine, v. 13.
dc.identifier.issn1472-6882
dc.identifier.urihttp://hdl.handle.net/11449/76087
dc.description.abstractBackground: Brown propolis is the major type of propolis found in Cuba; its principal component is nemorosone, the major constituent of Clusia rosea floral resins. Nemorosone has received increasing attention due to its strong in vitro anti-cancer action. The citotoxicity of nemorosone in several human cancer cell lines has been reported and correlated to the direct action it has on the estrogen receptor (ER). Breast cancer can be treated with agents that target estrogen-mediated signaling, such as antiestrogens. Phytoestrogen can mimic or modulate the actions of endogenous estrogens and the treatment of breast cancer with phytoestrogens may be a valid strategy, since they have shown anti-cancer activity.Methods: The aim of the present investigation was to assess the capacity of nemorosone to interact with ERs, by Recombinant Yeast Assay (RYA) and E-screen assays, and to determine by comet assay, if the compound causes DNA-damaging in tumoral and non-tumoral breast cells.Results: Nemorosone did not present estrogenic activity, however, it inhibited the 17-β-estradiol (E2) action when either of both methods was used, showing their antiestrogenicity. The DNA damage induced by the benzophenone in cancer and normal breast cells presented negative results.Conclusion: These findings suggest that nemorosone may have therapeutic application in the treatment of breast cancer. © 2013 Camargo et al.; licensee BioMed Central Ltd.en
dc.language.isoeng
dc.relation.ispartofBMC Complementary and Alternative Medicine
dc.sourceScopus
dc.subjectClusia rosea
dc.subjectEstrogenicity
dc.subjectGenotoxicity
dc.subjectNemorosone
dc.subjectPropolis
dc.subjectantiestrogen
dc.subjectantineoplastic agent
dc.subjectbenzophenone
dc.subjectestradiol
dc.subjectestrogen
dc.subjectestrogen receptor
dc.subjectnemorosone
dc.subjectpropolis
dc.subjectunclassified drug
dc.subjectbreast cancer
dc.subjectbreast cell
dc.subjectcomet assay
dc.subjectcontrolled study
dc.subjectCuba
dc.subjectDNA damage
dc.subjectdrug screening
dc.subjectgenotoxicity
dc.subjecthormone receptor interaction
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectBenzophenones
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectComet Assay
dc.subjectDNA Damage
dc.subjectDrug Evaluation, Preclinical
dc.subjectEstrogen Antagonists
dc.subjectEstrogens
dc.subjectHumans
dc.subjectMass Spectrometry
dc.subjectMutagens
dc.subjectPlant Extracts
dc.titleEvaluation of estrogenic, antiestrogenic and genotoxic activity of nemorosone, the major compound found in brown Cuban propolisen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Granada
dc.contributor.institutionUniversidad de La Habana
dc.description.affiliationUNESP- Univ. Estadual Paulista Faculty of Pharmaceutical Sciencies of Araraquara Department of Biological Sciences, Rodovia Araraquara-Jaú, Km 1, 14801-902 Araraquara, São Paulo
dc.description.affiliationSchool of Pharmacy University of Granada, Granada
dc.description.affiliationUNESP- Univ. Estadual Paulista Unidade São Vicente, Campus do Litoral Paulista, CEP 11330-900 São Vicente, São Paulo
dc.description.affiliationInstituto de Farmacia y Alimentos (IFAL) Universidad de La Habana, La Habana
dc.description.affiliationUnespUNESP- Univ. Estadual Paulista Faculty of Pharmaceutical Sciencies of Araraquara Department of Biological Sciences, Rodovia Araraquara-Jaú, Km 1, 14801-902 Araraquara, São Paulo
dc.description.affiliationUnespUNESP- Univ. Estadual Paulista Unidade São Vicente, Campus do Litoral Paulista, CEP 11330-900 São Vicente, São Paulo
dc.identifier.doi10.1186/1472-6882-13-201
dc.identifier.wosWOS:000322659300001
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-84880934126
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, São Vicentept
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
dc.identifier.file2-s2.0-84880934126.pdf
dc.identifier.lattes7927877224326837
dc.identifier.orcid0000-0003-3032-2556
unesp.author.lattes7927877224326837
unesp.author.orcid0000-0003-3032-2556[9]
dc.relation.ispartofjcr2.109
dc.relation.ispartofsjr0,858
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