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dc.contributor.authorSantiago, Maria Emília B. [UNESP]
dc.contributor.authorNeto, Luiz Silveira [UNESP]
dc.contributor.authorAlexandre, Eduardo Costa [UNESP]
dc.contributor.authorMunari, Danísio Prado [UNESP]
dc.contributor.authorAndrade, Mariana Macedo C. [UNESP]
dc.contributor.authorSomenzari, Marcos Arruda [UNESP]
dc.contributor.authorCiarlini, Paulo Cesar [UNESP]
dc.contributor.authorLima, Valéria Marçal Felix de [UNESP]
dc.date.accessioned2014-05-27T11:30:31Z
dc.date.available2014-05-27T11:30:31Z
dc.date.issued2013-09-01
dc.identifierhttp://dx.doi.org/10.1016/j.actatropica.2013.04.005
dc.identifier.citationActa Tropica, v. 127, n. 3, p. 174-180, 2013.
dc.identifier.issn0001-706X
dc.identifier.issn1873-6254
dc.identifier.urihttp://hdl.handle.net/11449/76390
dc.description.abstractThis study investigated the immunotherapeutic potential of the protein aggregate magnesium-ammonium phospholinoleate-palmitoleate anhydride immuno-modulator (P-MAPA) on canine visceral leishmaniasis. Twenty mongrel dogs presenting clinical symptoms compatible with leishmaniasis and diagnosis confirmed by the detection of anti-leishmania antibodies were studied. Ten dogs received 15 doses of the immunomodulator (2.0mg/kg) intramuscularly, and 10 received saline as a placebo. Skin and peripheral blood samples were collected following administration of the immunomodulator. The groups were followed to observe for clinical signals of remission; parasite load in the skin biopsies using real-time PCR, the cytokines IL-2, IL-10 and IFN-γ in the supernatant of peripheral blood mononuclear cells stimulated in vitro with either total promastigote antigen or phytohemagglutinin measured by capture ELISA, and changes in CD4+ and CD8+ T cell subpopulations evaluated by flow cytometry. Comparison between the groups showed that treatment with the immunomodulator promoted improvement in clinical signs and a significant reduction in parasite load in the skin. In peripheral blood mononuclear cell cultures, supernatants showed a decrease in IL-10 levels and an increase in IL-2 and IFN-γ. An increase in CD8+ T cells was observed in peripheral blood. In addition, the in vitro leishmanicidal action of P-MAPA was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and no leishmanicidal activity was detected. These findings suggest that P-MAPA has potential as an immunotherapeutic drug in canine visceral leishmaniasis, since it assists in reestablishing partial immunocompetence of infected dogs. © 2013 Elsevier B.V.en
dc.format.extent174-180
dc.language.isoeng
dc.relation.ispartofActa Tropica
dc.sourceScopus
dc.subjectCD4+ T
dc.subjectCD8+ T
dc.subjectLeishmania
dc.subjectP-MAPA
dc.subjectT lymphocytes
dc.subject3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
dc.subjectantileishmanial agent
dc.subjectgamma interferon
dc.subjectinterleukin 10
dc.subjectinterleukin 2
dc.subjectLeishmania antibody
dc.subjectphytohemagglutinin
dc.subjectplacebo
dc.subjectprotein aggregate magnesium ammonium phospholinoleate palmitoleate anhydride
dc.subjectprotozoal antigen
dc.subjectprotozoal protein
dc.subjectprotozoon antibody
dc.subjectunclassified drug
dc.subjectantibody
dc.subjectantigen
dc.subjectblood
dc.subjectcanid
dc.subjectdrug
dc.subjectimmunity
dc.subjectleishmaniasis
dc.subjectparasite
dc.subjectsymptom
dc.subjectanimal tissue
dc.subjectantibody detection
dc.subjectblood sampling
dc.subjectCD4+ T lymphocyte
dc.subjectCD8+ T lymphocyte
dc.subjectcell culture
dc.subjectcell stimulation
dc.subjectcellular immunity
dc.subjectclinical feature
dc.subjectcontrolled study
dc.subjectcytokine production
dc.subjectdog disease
dc.subjectenzyme linked immunosorbent assay
dc.subjectflow cytometry
dc.subjectimmunocompetence
dc.subjectimmunomodulation
dc.subjectimmunostimulation
dc.subjectimmunotherapy
dc.subjectin vitro study
dc.subjectmononuclear cell
dc.subjectnonhuman
dc.subjectparasite load
dc.subjectpromastigote
dc.subjectreal time polymerase chain reaction
dc.subjectremission
dc.subjectskin biopsy
dc.subjectsymptomatology
dc.subjectT lymphocyte subpopulation
dc.subjectvisceral leishmaniasis
dc.subjectCanis familiaris
dc.titleImprovement in clinical signs and cellular immunity of dogs with visceral leishmaniasis using the immunomodulator P-MAPAen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationFaculty of Agricultural and Veterinarian Sciences (FCAV) São Paulo State University (UNESP), Jaboticabal, SP
dc.description.affiliationFaculty of Veterinary Medicine UNESP, Araçatuba, SP
dc.description.affiliationDepartment of Clinical Surgery and Animal Reproduction Faculty of Veterinary Medicine, UNESP, Araçatuba, SP
dc.description.affiliationDepartment of Mathematical Sciences FCAV/UNESP, Jaboticabal, SP
dc.description.affiliationUnespFaculty of Agricultural and Veterinarian Sciences (FCAV) São Paulo State University (UNESP), Jaboticabal, SP
dc.description.affiliationUnespFaculty of Veterinary Medicine UNESP, Araçatuba, SP
dc.description.affiliationUnespDepartment of Clinical Surgery and Animal Reproduction Faculty of Veterinary Medicine, UNESP, Araçatuba, SP
dc.description.affiliationUnespDepartment of Mathematical Sciences FCAV/UNESP, Jaboticabal, SP
dc.identifier.doi10.1016/j.actatropica.2013.04.005
dc.identifier.wosWOS:000322294800004
dc.rights.accessRightsAcesso restrito
dc.identifier.scopus2-s2.0-84878395018
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatubapt
dc.identifier.lattes3613940018299500
dc.identifier.lattes4875352337860036
dc.identifier.orcid0000-0003-1480-5208
unesp.author.lattes3613940018299500[7]
unesp.author.lattes4875352337860036
unesp.author.orcid0000-0003-1480-5208[7]
dc.relation.ispartofjcr2.509
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