Biochemical responses in armored catfish (Pterygoplichthys anisitsi) after short-term exposure to diesel oil, pure biodiesel and biodiesel blends
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2013-09-01
Autores
Nogueira, Lílian [UNESP]
da Silva, Danilo Grünig Humberto [UNESP]
Oliveira, Thiago Yukio Kikuchi
da Rosa, Joel Maurício Correa
Felício, Andréia Arantes [UNESP]
de Almeida, Eduardo Alves [UNESP]
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Resumo
Biodiesel fuel is gradually replacing petroleum-based diesel oil use. Despite the biodiesel being considered friendlier to the environment, little is known about its effects in aquatic organisms. In this work we evaluated whether biodiesel exposure can affect oxidative stress parameters and biotransformation enzymes in armored catfish (Pterygoplichthys anisitsi, Loricariidae), a South American endemic species. Thus, fish were exposed for 2 and 7d to 0.01mLL-1 and 0.1mLL-1 of pure diesel, pure biodiesel (B100) and blends of diesel with 5% (B5) and 20% (B20) biodiesel. Lipid peroxidation (malondialdehyde) levels and the activities of the enzymes glutathione S-transferase, superoxide dismutase, catalase and glutathione peroxidase were measured in liver and gills. Also, DNA damage (8-oxo-7, 8-dihydro-2'-deoxyguanosine) levels in gills and 7-ethoxyresorufin-O-deethylase activity in liver were assessed. Pure diesel, B5 and B20 blends changed most of the enzymes tested and in some cases, B5 and B20 induced a higher enzyme activity than pure diesel. Antioxidant system activation in P. anisitsi was effective to counteract reactive oxygen species effects, since DNA damage and lipid peroxidation levels were maintained at basal levels after all treatments. However, fish gills exposed to B20 and B100 presented increased lipid peroxidation. Despite biodiesel being more biodegradable fuel that emits less greenhouse gases, the increased lipid peroxidation showed that biofuel and its blends also represent hazards to aquatic biota. © 2013 Elsevier Ltd.
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Biodiesel, Biomarker, Diesel oil, Oxidative stress, Pterygoplichthys anisitsi, 8-oxo-7 , 8-dihydro-2'- deoxyguanosine, Biotransformation enzymes, Glutathione peroxidase, Glutathione-S-transferase, Lipid peroxidation levels, Reactive oxygen species, Biomarkers, Damage detection, Diesel fuels, Enzyme activity, Fish, Greenhouse gases, Lipids, Oxygen, Toxicity, Biohazards, 8 hydroxyguanine, biodiesel, biological marker, catalase, diesel fuel, ethoxyresorufin deethylase, glutathione peroxidase, glutathione transferase, malonaldehyde, reactive oxygen metabolite, superoxide dismutase, antioxidant, aquatic organism, biochemical composition, biofuel, biomarker, diesel, enzyme activity, finfish, greenhouse gas, lipid, oxidation, physiological response, pollution effect, pollution exposure, biochemistry, biotransformation, catfish, DNA damage, endemic species, female, gill, lipid peroxidation, liver, male, nonhuman, oxidative stress, South America, Loricariidae, Pterygoplichthys
Como citar
Chemosphere, v. 93, n. 2, p. 311-319, 2013.