Subcutaneous tissue reaction and cytotoxicity of polyvinylidene fluoride and polyvinylidene fluoride-trifluoroethylene blends associated with natural polymers
Data de publicação2013-10-01
Direito de acesso
MetadadosExibir registro completo
Cytotoxicity and subcutaneous tissue reaction of innovative blends composed by polyvinylidene fluoride and polyvinylidene fluoride-trifluoroethylene associated with natural polymers (natural rubber and native starch) forming membranes were evaluated, aiming its applications associated with bone regeneration. Cytotoxicity was evaluated in mouse fibroblasts culture cells (NIH3T3) using trypan blue staining. Tissue response was in vivo evaluated by subcutaneous implantation of materials in rats, taking into account the presence of necrosis and connective tissue capsule around implanted materials after 7, 14, 21, 28, 35, 60, and 100 days of surgery. The pattern of inflammation was evaluated by histomorphometry of the inflammatory cells. Chemical and morphological changes of implanted materials after 60 and 100 days were evaluated by Fourier transform infrared (FTIR) absorption spectroscopy and scanning electron microscopy (SEM) images. Cytotoxicity tests indicated a good tolerance of the cells to the biomaterial. The in vivo tissue response of all studied materials showed normal inflammatory pattern, characterized by a reduction of polymorphonuclear leukocytes and an increase in mononuclear leukocytes over the time (p < 0.05 Kruskal-Wallis). On day 60, microscopic analysis showed regression of the chronic inflammatory process around all materials. FTIR showed no changes in chemical composition of materials due to implantation, whereas SEM demonstrated the delivery of starch in the medium. Therefore, the results of the tests performed in vitro and in vivo show that the innovative blends can further be used as biomaterials. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 1284-1293, 2013. Copyright © 2013 Wiley Periodicals, Inc.
Como citar este documento
Este item aparece nas seguintes coleções
Exibindo os itens relacionados pelo título, autor e palavra-chave.
A comparison of human and porcine acellular dermal tissues in the subcutaneous space of a rat model Hoyama, Erika ; Schellini, Silvana Artioli ; Marques, Mariângela Esther Alencar ; Rossa, Romualdo; Padovani, Carlos Roberto (Orbit, 2005) [Trabalho apresentado em evento]The study compared the host response to a human and a porcine acellular dermal tissue implanted in the subcutaneous space of a rat model. The human and porcine acellular grafts were surgically implanted in the subcutaneous ...
Treatment of gingival recession: Comparative study between subepithelial connective tissue graft and guided tissue regeneration Rosetti, Elizabeth Pimentel ; Marcantonio, Rosemary Adriana Chierici ; Rossa Júnior, Carlos ; Chaves, Eros S.; Goissis, Gilberto; Marcantonio Júnior, Elcio (Journal of Periodontology, 2000) [Artigo]Background: Various procedures have been proposed to treat gingival recession, but few studies compare these procedures to each other. The purpose of this study was to evaluate a clinical comparison of subepithelial ...
Porous polyethylene for tissue engineering applications in diabetic rats treated with calcitonin: Histomorphometric analysis Claro, Flávio Augusto; Lima, José Roberto Sá ; Salgado, Miguel Angel Castillo ; Gomes, Mônica Fernandes (International Journal of Oral and Maxillofacial Implants, 2005) [Artigo]Purpose: The purpose of this work was to study the bone tissue reaction after porous polyethylene (Polipore) implantation into surgical defects in the parietal bones of rats with streptozotocin-induced diabetes, treated ...