Glucogalactan: A polysaccharide isolated from the cell-wall of Verticillium Lecanii
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Data
2013-10-02
Autores
Corradi Da Silva, Maria L. [UNESP]
Exposti, Diego T.D. [UNESP]
Vasconcelos, Ana F.D. [UNESP]
Alexandre, Samara M.A. [UNESP]
Silveira, Joana L.M.
Ducatti, Diogo R.B.
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Resumo
A water-soluble polysaccharide was extracted with alkali from the cell wall of Verticillium lecanii (also called Lecanicillium lecanii). After freezing and thawing, the water-soluble fraction was purified by gel filtration chromatography on Sepharose CL-6B and eluted as one peak by HPSEC/RID. Monosaccharide analysis showed galactose and glucose (1.1:1), with traces of mannose (<1%). The structural characteristics were determined by spectroscopic analysis, FT-IR and 1D and 2D 1H and 13C NMR, and methylation results. On the basis of the data obtained, the following structure of the polysaccharide (E3SIV fraction) was established: where n ≈ 22 and m ≈ 22. © 2013 Elsevier Ltd. All rights reserved.
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Glucogalactan, Lecanicillium lecanii, NMR spectroscopy, Verticillium lecanii
Como citar
Carbohydrate Polymers, v. 98, n. 2, p. 1353-1359, 2013.