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dc.contributor.authorChaves de Souza, João Antônio [UNESP]
dc.contributor.authorNogueira, Andressa Vilas Boas [UNESP]
dc.contributor.authorChaves de Souza, Pedro Paulo [UNESP]
dc.contributor.authorKim, Yeon Jung
dc.contributor.authorSilva Lobo, Caroline [UNESP]
dc.contributor.authorPimentel Lopes de Oliveira, Guilherme José [UNESP]
dc.contributor.authorCirelli, Joni Augusto [UNESP]
dc.contributor.authorGarlet, Gustavo Pompermaier
dc.contributor.authorRossa, Carlos [UNESP]
dc.date.accessioned2014-05-27T11:30:50Z
dc.date.available2014-05-27T11:30:50Z
dc.date.issued2013-10-07
dc.identifierhttp://dx.doi.org/10.1155/2013/650812
dc.identifier.citationMediators of Inflammation, v. 2013.
dc.identifier.issn0962-9351
dc.identifier.issn1466-1861
dc.identifier.urihttp://hdl.handle.net/11449/76797
dc.description.abstractSOCS3 is an inducible endogenous negative regulator of JAK/STAT pathway, which is relevant in inflammatory conditions. We used a model of LPS-induced periodontal disease in rats to correlate SOCS3 expression with the inflammatory status. In vitro we used a murine macrophage cell line to assess the physical interaction between SOCS3 and STAT3 by coimmunoprecipitation. 30 ug of LPS from Escherichia coli were injected in the gingival tissues on the palatal aspect of first molars of the animals 3x/week for up to 4 weeks. Control animals were injected with the vehicle (PBS). The rats were sacrificed at 7, 15, and 30 days. Inflammation and gene expression were assessed by stereometric analysis, immunohistochemistry, RT-qPCR, and western blot. LPS injections increased inflammation, paralleled by an upregulation of SOCS3, of the proinflammatory cytokines IL-1β, IL-6, and TNF-and increased phosphorylation of STAT3 and p38 MAPK. SOCS3 expression accompanied the severity of inflammation and the expression of proinflammatory cytokines, as well as the activation status of STAT3 and p38 MAPK. LPS stimulation in a macrophage cell line in vitro induced transient STAT3 activation, which was inversely correlated with a dynamic physical interaction with SOCS3, suggesting that this may be a mechanism for SOCS3 regulatory function. © 2013 João Antônio Chaves de Souza et al.en
dc.language.isoeng
dc.relation.ispartofMediators of Inflammation
dc.sourceScopus
dc.titleSOCS3 expression correlates with severity of inflammation, expression of proinflammatory cytokines, and activation of STAT3 and p38 MAPK in LPS-induced inflammation in vivoen
dc.typeArtigo
dcterms.licensehttp://www.hindawi.com/journals/aaa/guidelines/
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Santo Amaro
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationDepartment of Diagnosis and Surgery School of Dentistry at Araraquara Universidade Estadual Paulista (UNESP), Rua Humaitá, 1680-Centro, 14801-903 Araraquara, SP
dc.description.affiliationDepartment of Physiology and Pathology School of Dentistry at Araraquara Universidade Estadual Paulista (UNESP), 14801-903 Araraquara, SP
dc.description.affiliationDepartment of Implantology University of Santo Amaro, 04743-030 Santo Amaro, SP
dc.description.affiliationDepartment of Biological Sciences School of Dentistry at Bauru University of São Paulo (USP), 17012-901 Bauru, SP
dc.description.affiliationUnespDepartment of Diagnosis and Surgery School of Dentistry at Araraquara Universidade Estadual Paulista (UNESP), Rua Humaitá, 1680-Centro, 14801-903 Araraquara, SP
dc.description.affiliationUnespDepartment of Physiology and Pathology School of Dentistry at Araraquara Universidade Estadual Paulista (UNESP), 14801-903 Araraquara, SP
dc.identifier.doi10.1155/2013/650812
dc.identifier.wosWOS:000324426000001
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-84884888723
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
dc.identifier.file2-s2.0-84884888723.pdf
dc.identifier.lattes2628593693450121
unesp.author.lattes2628593693450121
dc.relation.ispartofjcr3.549
dc.relation.ispartofsjr1,370
dc.relation.ispartofsjr1,370
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