eIF5A binds to translational machinery components and affects translation in yeast
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Data
2006-10-06
Autores
Zanelli, Cleslei Fernando [UNESP]
Maragno, Ana L. C.
Gregio, Ana P. B.
Komili, Suzanne
Pandolfi, Jose R.
Mestriner, Carlos A.
Lustri, Wilton R.
Valentini, Sandro Roberto [UNESP]
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Editor
Elsevier B.V.
Resumo
The putative translation factor eIF5A is essential for cell viability and is highly conserved from archebacteria to mammals. Although this protein was originally identified as a translation initiation factor, subsequent experiments did not support a role for eIF5A in general translation. In this work, we demonstrate that eIF-5A interacts with structural components of the 80S ribosome, as well as with the translation elongation factor 2 (eEF2). Moreover, eIF5A is further shown to cofractionate with monosomes in a translation-dependent manner. Finally, eIF5A mutants show altered polysome profiles and are sensitive to translation inhibitors. Our results re-establish a function for eIF5A in translation and suggest a role for this factor in translation elongation instead of translation initiation. (c) 2006 Elsevier B.V. All rights reserved.
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Palavras-chave
eIF5A, ribosome, translation, eEF2, P0, L11, hypusine, protein synthesis
Como citar
Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 348, n. 4, p. 1358-1366, 2006.