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dc.contributor.authorPaneto, Greiciane G. [UNESP]
dc.contributor.authorLongo, Larissa V. G. [UNESP]
dc.contributor.authorMartins, Joyce A. [UNESP]
dc.contributor.authorde Camargo, Maria Angelica [UNESP]
dc.contributor.authorCosta, Jeane C. [UNESP]
dc.contributor.authorde Mello, Aline C. O. [UNESP]
dc.contributor.authorChen, Bety
dc.contributor.authorOliveira, Rogerio N.
dc.contributor.authorHirata, Mario H.
dc.contributor.authorCicarelli, Regina Maria Barretto [UNESP]
dc.identifier.citationJournal of Forensic Sciences. Malden: Wiley-blackwell Publishing, Inc, v. 55, n. 3, p. 715-718, 2010.
dc.description.abstractMitochondrial DNA (mtDNA) analysis has proved useful for forensic identification especially in cases where nuclear DNA is not available, such as with hair evidence. Heteroplasmy, the presence of more than one type of mtDNA in one individual, is a common situation often reported in the first and second mtDNA hypervariable regions (HV1/HV2), particularly in hair samples. However, there is no data about heteroplasmy frequency in the third mtDNA hypervariable region (HV3). To investigate possible heteroplasmy hotspots, HV3 from hair and blood samples of 100 individuals were sequenced and compared. No point heteroplasmy was observed, but length heteroplasmy was, both in C-stretch and CA repeat. To observe which CA "alleles" were present in each tissue, PCR products were cloned and re-sequenced. However, no variation among CA alleles was observed. Regarding forensic practice, we conclude that point heteroplasmy in HV3 is not as frequent as in the HV1/HV2.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.publisherWiley-Blackwell Publishing, Inc
dc.relation.ispartofJournal of Forensic Sciences
dc.sourceWeb of Science
dc.subjectforensic scienceen
dc.subjectmitochondrial DNAen
dc.titleHeteroplasmy in Hair: Study of Mitochondrial DNA Third Hypervariable Region in Hair and Blood Samplesen
dcterms.rightsHolderWiley-blackwell Publishing, Inc
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationSão Paulo State Univ, Sch Pharmaceut Sci, UNESP, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv São Paulo, Sch Dent, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Pharmaceut Sci, BR-05508900 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Sch Pharmaceut Sci, UNESP, BR-14801902 Araraquara, SP, Brazil
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 05/03984-5
dc.description.sponsorshipIdFAPESP: 06/06772-1
dc.description.sponsorshipIdCNPq: 2007-0/140052
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
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