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dc.contributor.authorMartins, Carlos Henrique Gomes
dc.contributor.authorBauab, Taís Maria [UNESP]
dc.contributor.authorLeite, Clarice Queico Fujimura [UNESP]
dc.contributor.authorFalcão, Deise Pasetto [UNESP]
dc.date.accessioned2014-05-20T13:25:21Z
dc.date.available2014-05-20T13:25:21Z
dc.date.issued2007-08-01
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762007005000072
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 5, p. 587-592, 2007.
dc.identifier.issn0074-0276
dc.identifier.urihttp://hdl.handle.net/11449/8022
dc.description.abstractRibotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O:1a and O:3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of extracted DNA with the restriction endonucleases SmaI and PstI, separation by electrophoresis and hybridization with a digoxigenin-labeled cDNA probe. The presence of the chromosomal virulence marker genes inv, irp1, irp2, psn, ybtE, ybtP-ybtQ, and ybtX-ybtS, of the IS100 insertion sequence, and of the plasmid gene lcrF was detected by polymerase chain reaction. The strains were grouped into four distinct ribotypes, all of them comprising several strains. Ribotypes 1 and 4 presented distinct profiles, with 57.3% genetic similarity, ribotypes 2 and 3 presented 52.5% genetic similarity, and genetic similarity was 45% between these two groups (1/4 and 2/3). All strains possessed the inv, irp1, and irp2 genes. Additionally, strains of serogroup O:1a carried psn, ybtE, ybtP-ybtQ, ybtX-ybtS, and IS100. As expected lcrF was only detected in strains harboring the virulence plasmid. These data demonstrate the presence of Y. pseudotuberculosis strains harboring genotypic virulence markers in the livestock from Southern Brazil and that the dissemination of these bacteria may occur between herds.en
dc.format.extent587-592
dc.language.isoeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz
dc.sourceSciELO
dc.subjectribotypingen
dc.subjectYersinia pseudotuberculosisen
dc.subjectvirulence markersen
dc.subjectpolymerase chain reactionen
dc.titleRibotyping and virulence markers of Yersinia pseudotuberculosis strains isolated from animals in Brazilen
dc.typeArtigo
dc.contributor.institutionUniversidade de Franca Laboratório de Pesquisa em Microbiologia Aplicada
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationUniversidade de Franca Laboratório de Pesquisa em Microbiologia Aplicada
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências Biológicas
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências Biológicas
dc.identifier.doi10.1590/S0074-02762007005000072
dc.identifier.scieloS0074-02762007000500008
dc.rights.accessRightsAcesso aberto
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
dc.identifier.fileS0074-02762007000500008.pdf
dc.identifier.lattes4910754838277580
dc.identifier.lattes2114570774349859
unesp.author.lattes4910754838277580
unesp.author.lattes2114570774349859
dc.relation.ispartofjcr2.833
dc.relation.ispartofsjr1,172
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