Performance characteristics of high performance liquid chromatography, first order derivative UV spectrophotometry and bioassay for fluconazole determination in capsules
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Data
2012-01-01
Autores
Corrêa, Josilene Chaves Ruela [UNESP]
Chin, Chung Man [UNESP]
Salgado, Hérida Regina Nunes [UNESP]
Vianna-Soares, Cristina Duarte
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Química
Resumo
The bioassay, first order derivative UV spectrophotometry and chromatographic methods for assaying fluconazole capsules were compared. They have shown great advantages over the earlier published methods. Using the first order derivative, the UV spectrophotometry method does not suffer interference of excipients. Validation parameters such as linearity, precision, accuracy, limit of detection and limit of quantitation were determined. All methods were linear and reliable within acceptable limits for antibiotic pharmaceutical preparations being accurate, precise and reproducible. The application of each method as a routine analysis should be investigated considering cost, simplicity, equipment, solvents, speed, and application to large or small workloads.
Descrição
Palavras-chave
fluconazole, liquid chromatography, bioassay
Como citar
Química Nova. Sociedade Brasileira de Química, v. 35, n. 3, p. 530-534, 2012.