Stereoselective analysis of carvedilol in human plasma and urine using HPLC after chiral derivatization
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Data
2008-07-01
Autores
Peccinini, Rosangela Goncalves [UNESP]
Ximenes, Valdecir Farias [UNESP]
Cesarino, Evandro J.
Lanchote, Vera L.
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Editor
John Wiley & Sons Ltd
Resumo
An enantioselective high-performance liquid chromatographic method for the analysis of carvedilol in plasma and urine was developed and validated using (-)-menthyl chloroformate (MCF) as a derivatizing reagent. Chloroform was used for extraction, and analysis was performed by HPLC on a C18 column with a fluorescence detector. The quantitation limit was 0.25 ng/ml for S(-)-carvedilol in plasma and 0.5 ng/ml for R(+)-carvedilol in plasma and for both enantiomers in urine. The method was applied to the study of enantioselectivity in the pharmacokinetics of carvedilol administered in a multiple dose regimen (25mg/12h) to a hypertensive elderly female patient. The data obtained demonstrated highest plasma levels for the R(+)-carvedilol(AUCSS 75.64 vs 37.29ng/ml). The enantiomeric ratio R(+)/S(-) was 2.03 for plasma and 1.49 0 - 12 for urine (Aeo-12 17.4 vs 11.7 pg). Copyright (c) 2008 John Wiley & Sons, Ltd.
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carvedilol, enantiomers, HPLC, pharmacokinetics
Como citar
Biopharmaceutics & Drug Disposition. Chichester: John Wiley & Sons Ltd, v. 29, n. 5, p. 280-288, 2008.