Absorção e acúmulo de manganês em soja RR sob efeito do glyphosate
Carregando...
Data
2010-04-12
Autores
Andrade, Gabriel José Massoni de [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
Frente a dúvida sobre a nutrição da soja com manganês influenciada pelo glyphosate e a inclusão do gene de resistência, objetivou-se estudar o acúmulo e distribuição do nutriente na planta de soja geneticamente modificada sob efeito da aplicação de glyphosate, assim como a cinética de absorção do Mn pela soja transgênica sob ação do mesmo herbicida. Foram conduzidos dois experimentos em casa de vegetação no Departamento de Produção Vegetal - Agricultura da Faculdade de Ciências Agronômicas, UNESP-Botucatu. Experimento 1: Em delineamento de blocos completos ao acaso e em esquema fatorial 5 x 3, com quatro repetições, a absorção e o acúmulo de Mn foram avaliados em duas cultivares de soja quase isogênicas (Conquista e Valiosa RR), cultivadas em solução nutritiva. Foram empregados os seguintes níveis de Mn à solução nutritiva: 0; 0,085; 0,125; 0,250; 0,500 mg L-1. Após 25 dias de cultivo as plantas de soja transgênica, destinadas ao estudo sob efeito do glyphosate, foram submetidas à aplicação do herbicida, formulado a base do sal de isopropilamina, via foliar, na dose de 720 g e.a. ha-1. Todas as plantas de soja, convencional e transgênica, com e sem a adição do herbicida, foram coletadas na mesma data (15 dias após a aplicação do herbicida). Realizaram-se divisões das plantas em raízes, hastes e folhas, para a determinação da massa de matéria seca (MMS) e os teores de Mn. Experimento 2: O estudo da cinética de absorção do Mn pela cultivar Valiosa RR foi organizado em delineamento de blocos completos ao acaso, com sete repetições. As plantas foram submetidas ao glyphosate aos 26 dias de cultivo, o qual foi aplicado via foliar, com a mesma formulação do experimento anterior, nas doses de 0 (zero), 15 e 960 g e.a. ha-1. Utilizando-se da técnica de exaustão do Mn na solução, após quatro dias da aplicação do herbicida foi realizado...
Faced with questions about the nutrition of soybean with manganese influenced by glyphosate and the inclusion of the resistance gene, it was aimed to study the accumulation and partition of nutrient in soybean plant genetically modified under effect of glyphosate and the kinetics of Mn absorption by soybean GM under the action of the same herbicide. It was run two experiments in a greenhouse at the Department of Plant Production, Faculty of Agronomic Sciences, UNESP-Botucatu. Experiment 1: In a randomized complete block design and in a 5 x 3 factorial scheme, with four replications, the absorption and accumulation of Mn was evaluated in two cultivars of soybean near-isogenic (Conquista and Valiosa GR), that was grown in nutrient solution. The following Mn levels of the nutrient solution were utilized: 0, 0.085, 0.125, 0.250, 0.500 mg L-1. After 25 days of GR soybean plants cultivation, the leaves of soybean were sprayed with glyphosate formulations on the basis of isopropylamine salt using 720 g a.e. ha-1. All soybean plants, conventional, GR, with and without glyphosate, were collected on the same date (15 days after herbicide application). Then they were separated by roots, stems and leaves, for the determination of dry matter and Mn concentration. Experiment 2: The experiment was randomized using complete block design with seven replicates studying the kinetics of Mn uptake by GR Valiosa soybean cultivar under doses of glyphosate. After 26 days of soybean cultivation the glyphosate was applied with the same wording of the previous experiment, using doses of 0 (zero), 15 and 960 g a.e. ha-1 doses. Using the technique of Mn exhaustion from the nutrient solution after four days of herbicide application, it was carried out a study of the kinetics of Mn absorption. Righ after the end of the experiment of exhaustion, the plants were harvested and separated in leaves, stems and roots for... (Complete abstract click electronic access below)
Faced with questions about the nutrition of soybean with manganese influenced by glyphosate and the inclusion of the resistance gene, it was aimed to study the accumulation and partition of nutrient in soybean plant genetically modified under effect of glyphosate and the kinetics of Mn absorption by soybean GM under the action of the same herbicide. It was run two experiments in a greenhouse at the Department of Plant Production, Faculty of Agronomic Sciences, UNESP-Botucatu. Experiment 1: In a randomized complete block design and in a 5 x 3 factorial scheme, with four replications, the absorption and accumulation of Mn was evaluated in two cultivars of soybean near-isogenic (Conquista and Valiosa GR), that was grown in nutrient solution. The following Mn levels of the nutrient solution were utilized: 0, 0.085, 0.125, 0.250, 0.500 mg L-1. After 25 days of GR soybean plants cultivation, the leaves of soybean were sprayed with glyphosate formulations on the basis of isopropylamine salt using 720 g a.e. ha-1. All soybean plants, conventional, GR, with and without glyphosate, were collected on the same date (15 days after herbicide application). Then they were separated by roots, stems and leaves, for the determination of dry matter and Mn concentration. Experiment 2: The experiment was randomized using complete block design with seven replicates studying the kinetics of Mn uptake by GR Valiosa soybean cultivar under doses of glyphosate. After 26 days of soybean cultivation the glyphosate was applied with the same wording of the previous experiment, using doses of 0 (zero), 15 and 960 g a.e. ha-1 doses. Using the technique of Mn exhaustion from the nutrient solution after four days of herbicide application, it was carried out a study of the kinetics of Mn absorption. Righ after the end of the experiment of exhaustion, the plants were harvested and separated in leaves, stems and roots for... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Soja, Plantas - Nutrição mineral, Elementos traços na nutrição, Cinetica de absorção, Glycine Max L, Absorption kinetics, Micronutrients, Plant nutrition
Como citar
ANDRADE, Gabriel José Massoni de. Absorção e acúmulo de manganês em soja RR sob efeito do glyphosate. 2010. ix, 57 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agronômicas de Botucatu, 2010.