Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi
Data
2011-11-01
Autores
Pelizzaro-Rocha, Karin Juliane
Veiga-Santos, Phercyles
Lazarin-Bidoia, Danielle
Ueda-Nakamura, Tania
Dias Filho, Benedito Prado
Ximenes, Valdecir Farias [UNESP]
Silva, Sueli Oliveira
Nakamura, Celso Vataru
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Because of its severe side effects and variable efficacy, the current treatment for Chagas disease is unsatisfactory. Natural compounds are good alternative chemotherapeutic agents for the treatment of this infection. Recently, our group reported the antiproliferative activity and morphological alterations in epimastigotes and intracellular amastigotes of Trypanosoma cruzi treated with eupomatenoid-5, a neolignan isolated from leaves of Piper regnellii var. pallescens. Here, we demonstrate that eupomatenoid-5 exhibited activity against trypomastigotes, the infective form of T cruzi (EC(50) 40.5 mu W), leading to ultrastructural alteration and lipoperoxidation in the cell membrane. Additionally, eupomatenoid-5 induced depolarization of the mitochondrial membrane, lipoperoxidation and increased G6PD activity in epimastigotes of T cruzi. These findings support the possibility that different mechanisms may be targeted, according to the form of the parasite, and that the plasma membrane and mitochondria are the structures that are most affected in trypomastigotes and epimastigotes, respectively. Thus, the trypanocidal action of eupomatenoid-5 may be associated with mitochondrial dysfunction and oxidative damage, which can trigger destructive effects on biological molecules of T cruzi, leading to parasite death. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Descrição
Palavras-chave
Trypanosoma cruzi, Eupomatenoid-5, Antioxidant system
Como citar
Microbes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 12-13, p. 1018-1024, 2011.