Avaliação histológica e microbiológica de curativos de demora à base de hidróxido de cálcio usados em endodontia
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Data
2011-03-30
Autores
Andolfatto, Carolina [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Este estudo objetivou avaliar a biocompatibilidade, a atividade antimicrobiana e o pH de curativos de demora à base de hidróxido de cálcio usados em endodontia. A análise da biocompatibilidade foi realizada no tecido conjuntivo subcutâneo de ratos. Nesta metodologia, três medicações intracanal à base de hidróxido de cálcio Calen, UltraCal XS e Hydropast após serem colocadas separadamente em tubos de silicone, foram inseridos no subcutâneo de ratos. Os 48 ratos (Rattus Norvegicus Holtzman) foram distribuídos aleatoriamente em três grupos: Grupo Calen (GC), Grupo UltraCal XS (GU) e grupo Hydropast (GH). Após os períodos de 7 e 30 dias estes animais foram mortos, as peças obtidas processadas e realizada análise morfológica e morfométrica. No teste de difusão em ágar com a confecção de poços, cinco curativos de demora, Calen, Calen PMCC, Hydropast, Hydropasi+I e UtraCal XS foram expostos aos microrganismos Micrococcus luteus (ATCC 9341), Enterococcus faecalis (ATCC 29122), Pseudomonas aeruginosa (ATCC 27853) e Candida albicans (ATCC 10231) para avaliar o poder antimicrobiano destas pastas. E, por fim, a análise do pH foi realizada preenchendo tubos de polivinil (PVC), 10 para cada curativo de demora, Calen, Calen PMCC, Hydropast, Hydropast+I e UltraCal XS. Em seguida, foram mergulhados em frascos plásticos contendo 10 ml de água deionizada, cujo pH foi aferido previamente. Os frascos foram fechados e mantidos em uma estufa a 37°C. Após 12, 24, 48 e 72 horas, o pH das soluções contidas nos frascos plásticos foi medido com auxílio do peagâmetro DMPH-2. De acordo com as metodologias empregadas, os resultados obtidos no teste de biocompatibilidade, por meio de análises morfológicas e morfométricas mostraram, inicialmente, severa resposta inflamatória nos...
This study aimed to evaluate the biocompatibility, the antibacterial activity and the pH of intracanal medicaments based on of calcium hydroxide used in endodontics. The analysis of the biocompatibility was performed in the subcutaneous tissue of rats. In the methodology, three intracanal medications to the base of calcium hydroxide: Calen, UltraCal XS and Hydropast were placed in separate silicone tubes and then inserted into the subcutaneous tissue of rats. The 48 rats (Rattus Norvegicus Holtzman) were randomly and equally distributed into three groups as follows: Calen group (CG), UltraCal XS group (UG) and Hydropast group (HG). After periods of 7 and 30 days these animals were dead and the specimens were processed and in the end the morphological and morphometric analysis were done. In the agar diffusion method with the construction of wells, five intracanal medicaments (Calen, Calen PMCC, Hydropast, Hidropast + I and UtraCal XS) were exposed to the microorganisms: Micrococcus luteus (ATCC 9341), Enterococcus faecalis (ATCC 29122), Pseudomonas aeruginosa (ATCC 27853) and Candida albicans (ATCC 10231) to evaluate the antimicrobial power of these pastes. And finally, the pH analysis was performed by filling tubes of polyvinyl (PVC), 10 for each intracal medication with Calen, Calen PMCC, Hydropast, Hydropast + I and UltraCal XS. Were then immersed in plastic vials containing 10 ml of deionized water, whose pH was previously measured. The vials were sealed and kept in an incubator at 37°C. After 12, 24, 48 and 72 hours, the pH of the solutions contained in plastic bottles was measured on the pH meter DMPH-2. According to the methodologies employed, the biocompatibility tests results by morphometrical and morphological analysis, initially, showed a severe inflammatory response in tissues... (Complete abstract click electronic access below)
This study aimed to evaluate the biocompatibility, the antibacterial activity and the pH of intracanal medicaments based on of calcium hydroxide used in endodontics. The analysis of the biocompatibility was performed in the subcutaneous tissue of rats. In the methodology, three intracanal medications to the base of calcium hydroxide: Calen, UltraCal XS and Hydropast were placed in separate silicone tubes and then inserted into the subcutaneous tissue of rats. The 48 rats (Rattus Norvegicus Holtzman) were randomly and equally distributed into three groups as follows: Calen group (CG), UltraCal XS group (UG) and Hydropast group (HG). After periods of 7 and 30 days these animals were dead and the specimens were processed and in the end the morphological and morphometric analysis were done. In the agar diffusion method with the construction of wells, five intracanal medicaments (Calen, Calen PMCC, Hydropast, Hidropast + I and UtraCal XS) were exposed to the microorganisms: Micrococcus luteus (ATCC 9341), Enterococcus faecalis (ATCC 29122), Pseudomonas aeruginosa (ATCC 27853) and Candida albicans (ATCC 10231) to evaluate the antimicrobial power of these pastes. And finally, the pH analysis was performed by filling tubes of polyvinyl (PVC), 10 for each intracal medication with Calen, Calen PMCC, Hydropast, Hydropast + I and UltraCal XS. Were then immersed in plastic vials containing 10 ml of deionized water, whose pH was previously measured. The vials were sealed and kept in an incubator at 37°C. After 12, 24, 48 and 72 hours, the pH of the solutions contained in plastic bottles was measured on the pH meter DMPH-2. According to the methodologies employed, the biocompatibility tests results by morphometrical and morphological analysis, initially, showed a severe inflammatory response in tissues... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Hidroxido de calcio, Endodontia, Endodontics, Calcium hydroxide
Como citar
ANDOLFATTO, Carolina. Avaliação histológica e microbiológica de curativos de demora à base de hidróxido de cálcio usados em endodontia. 2011. 87 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araraquara, 2011.