Identificação da bactéria endossimbionte Wolbachia em populações de moscas-das-frutas do complexo Anastrepha fraterculus (Diptera: Tephritidae)
Carregando...
Arquivos
Data
2009-08-28
Autores
Marcon, Helena Sanches [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
Wolbachia é uma bactéria endossimbionte comumente encontrada nos tecidos reprodutores de invertebrados, sendo herdada vertical e horizontalmente. Esta bactéria é desencadeadora de inúmeras alterações reprodutivas, dentre elas a incompatibilidade citoplasmática. Bactéria Wolbachia apresenta oito diferentes tipos de genoma, identificados de A a H. Dentre os hospedeiros da Wolbachia estão as moscas-das-frutas do gênero Anastrepha, que são um importante inseto-praga causador de inúmeras perdas na fruticultura de vários países na América. Neste estudo, foram utilizados os primers 16S rDNA, ftsZ e wsp na detecção da Wolbachia e identificação do supergrupo em três populações de Anastrepha do complexo fraterculus, de diferentes regiões do estado de São Paulo. Em todas as amostras de moscas foi detectada a presença da Wolbachia do supergrupo A, através da utilização dos primers 16S rDNA e wsp, visto que o ftsZ apresentou baixa sensibilidade na detecção desta bactéria em Anastrepha. Comparações das sequências do gene wsp dos 62 indivíduos no Genebank possibilitaram a identificação de duas linhagens de Wolbachia, uma pertencente a Anastrepha sp. 2 (wAsp2B) e a outra ao nematóide Brugia pahangi (Bp-1-1001). Com isso, sugere-se a ocorrência de transferência horizontal da linhagem Bp-1-1001 em Anastrepha através das vespas parasitas de dípteros. A partir de comparações entre as sequências geradas com o gene wsp, observou-se a ocorrência de quatro diferentes sequências pertencentes a novas linhagens de Wolbachia, denominadas wAsc, wAnc, wBjc e wBsp. Essas linhagens estão distribuídas nas diferentes populações de moscas-das-frutas do gênero Anastrepha
Wolbachia is a endosymbiont bacteria commonly found in reproducer tissue of invertebrates, being vertically and horizontally inherited. This bacteria cause innumerable reproductive alterations, among them a cytoplasmic incompatibility. Wolbachia have eight different types of genome, designated from A to H. Among Wolbachia host there are the fruit flies of genus Anastrepha. These arthropods are important pest insect that causes many losses in fruit production of many countries of America. In this work, were used the primers 16S rDNA, ftsZ and wsp to detect Wolbachia and to identify the supergroups of three Anastrepha populations the different regions of São Paulo state, Brazil. In all fruit flies samples, it was detected the presence of Wolbachia of the A supergroup, through the use of primers 16S rDNA and wsp, since ftsZ has low sensitivity in detecting this bacteria in Anastrepha. Comparisons of the wsp gene sequences of 62 samples in GeneBank, allowed the identification of two Wolbachia lineages, one relating to Anastrepha sp. 2 (wAsp2B) and other in Brugia pahangi nematode (Bp-1-1001). Therefore it is propose the occurrence the horizontal transference of Wolbachia lineages Bp-1- 1001 into Anastrepha through the did parasite wasps of dipterous. The comparisons among the wsp gene sequences showed the occurrence of four different sequences, possibly belonging to the news lineages of Wolbachia, which were named wAsc, wAnc, wBjc and wBsp. These lineages are distributed in different fruit flies of populations of the Anastrepha genus
Wolbachia is a endosymbiont bacteria commonly found in reproducer tissue of invertebrates, being vertically and horizontally inherited. This bacteria cause innumerable reproductive alterations, among them a cytoplasmic incompatibility. Wolbachia have eight different types of genome, designated from A to H. Among Wolbachia host there are the fruit flies of genus Anastrepha. These arthropods are important pest insect that causes many losses in fruit production of many countries of America. In this work, were used the primers 16S rDNA, ftsZ and wsp to detect Wolbachia and to identify the supergroups of three Anastrepha populations the different regions of São Paulo state, Brazil. In all fruit flies samples, it was detected the presence of Wolbachia of the A supergroup, through the use of primers 16S rDNA and wsp, since ftsZ has low sensitivity in detecting this bacteria in Anastrepha. Comparisons of the wsp gene sequences of 62 samples in GeneBank, allowed the identification of two Wolbachia lineages, one relating to Anastrepha sp. 2 (wAsp2B) and other in Brugia pahangi nematode (Bp-1-1001). Therefore it is propose the occurrence the horizontal transference of Wolbachia lineages Bp-1- 1001 into Anastrepha through the did parasite wasps of dipterous. The comparisons among the wsp gene sequences showed the occurrence of four different sequences, possibly belonging to the news lineages of Wolbachia, which were named wAsc, wAnc, wBjc and wBsp. These lineages are distributed in different fruit flies of populations of the Anastrepha genus
Descrição
Palavras-chave
Moscas-das-frutas, Bactéria, Wolbachia, Anastrepha, Sensitivity
Como citar
MARCON, Helena Sanches. Identificação da bactéria endossimbionte Wolbachia em populações de moscas-das-frutas do complexo Anastrepha fraterculus (Diptera: Tephritidae). 2009. 110 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências de Botucatu, 2009.