Expressão de genes relacionados à função mitocondrial em bovinos Nelore extremos para consumo alimentar residual
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Data
2013-02-22
Autores
Fonseca, Larissa Fernanda Simielli [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A pecuária de corte é uma atividade de grande importância social e econômica no Brasil e criar animais mais produtivos e eficientes é fundamental para aumentar a rentabilidade do sistema. Um dos componentes de maior custo envolvido na produção animal é a alimentação. Por isso, muitos índices já foram propostos com o intuito de melhorar a eficiência alimentar. Uma das características que têm sido estudadas, com objetivo de selecionar animais, é o consumo alimentar residual (CAR), que é estimado considerandose a diferença entre o consumo real e a quantidade de alimento que o animal deveria ingerir baseado no seu peso vivo médio, o que permite identificar e selecionar animais mais eficientes, sem que haja seleção para maior peso a idade adulta. A função mitocondrial tem sido apontada como sendo um fator de grande influência no CAR. Com isso, analisar genes envolvidos na função mitocondrial torna-se uma alternativa para identificar marcadores moleculares associados à maior eficiência alimentar. O co-ativador PGC1, pode induzir a biogênese mitocondrial, termogênese e respiração. A proteína TFAM (fator de transcrição mitocondrial A) está envolvida no processo de transcrição do DNA mitocondrial e é um fator-chave para a replicação do DNA mitocondrial. As proteínas desacopladoras (UCPs) localizam-se na membrana interna da mitocôndria, e dentre elas, estão a UCP2 e a UCP3, que atuam no controle da termogênese adaptativa e da produção de espécies reativas de oxigênio na mitocôndria. Nesse trabalho foram analisadas a expressão dos genes PGC1 , TFAM, UCP2 e UCP3 por meio da técnica de PCR quantitativa em tempo real, em tecidos hepático e muscular de dois grupos de bovinos Nelore com valores contrastantes de CAR, visando avaliar as relações destes genes com consumo alimentar residual...
The beef cattle industry is an activity of significative social and economic importance in Brazil and to raise more efficient animals is fundamental to increase the system profitability . One of the most expensive components involved in animal production is the cost of food. Therefore, many indexes have been proposed to improve feed efficiency. One of the traits that have been studied in order to select animals is the residual feed intake (RFI), which is estimated considering the difference between the real consumption and the amount of food the animal should eat based on its live weight, which allows to identify and select more efficient animals, without selecting for higher adult weight. Mitochondrial function has been identified as an influential factor on RFI. Thus, to analyze genes involved in mitochondrial function becomes an alternative to identify molecular markers associated with higher feed efficiency. The co-activator PGC1 can induce mitochondrial biogenesis, thermogenesis and breathing. The protein TFAM (mitochondrial transcription factor A) is involved in mitochondrial DNA transcription and is a key factor for the replication of mitochondrial DNA. The uncoupling proteins (UCPs) are located in the inner mitochondrial membrane, and among them, are UCP2 and UCP3, which act in the control of adaptive thermogenesis and of the production of reactive oxygen species in the mitochondria. In this study the expression of genes PGC1 , TFAM, UCP2 and UCP3 by real-time quantitative PCR in liver and muscle tissues in two groups of Nellore cattle with contrasting values of RFI were analyzed, to evaluate the relationships of these genes with residual feed intake. In liver tissue, the TFAM and UCP2 genes were more expressed in negative RFI animals compared to positive RFI animals. The PGC1 gene expression had no significant difference... (Complete abstract click electronic access below)
The beef cattle industry is an activity of significative social and economic importance in Brazil and to raise more efficient animals is fundamental to increase the system profitability . One of the most expensive components involved in animal production is the cost of food. Therefore, many indexes have been proposed to improve feed efficiency. One of the traits that have been studied in order to select animals is the residual feed intake (RFI), which is estimated considering the difference between the real consumption and the amount of food the animal should eat based on its live weight, which allows to identify and select more efficient animals, without selecting for higher adult weight. Mitochondrial function has been identified as an influential factor on RFI. Thus, to analyze genes involved in mitochondrial function becomes an alternative to identify molecular markers associated with higher feed efficiency. The co-activator PGC1 can induce mitochondrial biogenesis, thermogenesis and breathing. The protein TFAM (mitochondrial transcription factor A) is involved in mitochondrial DNA transcription and is a key factor for the replication of mitochondrial DNA. The uncoupling proteins (UCPs) are located in the inner mitochondrial membrane, and among them, are UCP2 and UCP3, which act in the control of adaptive thermogenesis and of the production of reactive oxygen species in the mitochondria. In this study the expression of genes PGC1 , TFAM, UCP2 and UCP3 by real-time quantitative PCR in liver and muscle tissues in two groups of Nellore cattle with contrasting values of RFI were analyzed, to evaluate the relationships of these genes with residual feed intake. In liver tissue, the TFAM and UCP2 genes were more expressed in negative RFI animals compared to positive RFI animals. The PGC1 gene expression had no significant difference... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Bovino de corte, Nelore (Zebu), Alimentos - Consumo, Reação em cadeia de polimerase, Marcadores biologicos, Genes, Proteínas, Proteins
Como citar
FONSECA, Larissa Fernanda Simielli. Expressão de genes relacionados à função mitocondrial em bovinos Nelore extremos para consumo alimentar residual. 2013. xii, 49 p. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias, 2013.