Estudo da atividade antidermatofitica de Protocatecuatos contra T. rubrum e T. interdigitale
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Data
2011-11-23
Autores
Soares, Luciana Arantes [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
São evidentes a necessidade de novas substâncias antifúngicas e a grande importância dos produtos naturais no desenvolvimento de novas ferramentas terapêuticas. Assim, neste estudo avaliou-se a atividade antifúngica dos derivados sintéticos esterificados do ácido protocatecuico. Todos os isolados apresentaram bons resultados ao teste de suscetibilidade aos derivados sintéticos do ácido protocatecuico demonstrando que 62,5% foram ativos em relação para dois isolados de T. rubrum (Tr1 e Tr2), T. rubrum MYA 3108 (Tr3) e isolado clínico T. interdigitale (Tm1) e 68,7%, para isolado clínico T. interdigitale (Tm2) e T. interdigitale ATCC 40131 (Tm3) dos 16 compostos testados. Como referência para avaliação da Concentração Inibitória Mínima (CIM) foram considerados todos os resultados com valores ≤ 62,5 ug\mL. A combinação fluconazol mais protocatecuatos de pentila, hexila, heptila, octila e nonila foi aditivo para isolados clínicos Tr1, Tm1 e Tm3, exceto a combinação com fluconazol mais protocatecuato de heptila, que revelou um efeito sinérgico contra Tm3. A griseofulvina em combinação com protocatecuato de nonila mostrou atividade sinérgica em duas linhagens de dermatófitos, atividade aditiva para quatro linhagens. Oito derivados do ácido protocatecuico foram associados quimicamente com o miconazol sendo encontradas interações sinérgicas e aditivas. Foi testada a associação entre protocatecuatos e o antifúngico nistatina, resultando interações antagônicas, demonstrando assim que o polieno...
There is a clear need for new antifungal compounds, and the importance of natural products in the development of novel therapeutic tools. In this study, it was evaluated the antifungal activity of protocatechuic acid esters. All of the fungus isolated showed good results when testing susceptibility to synthetic derivatives of protocatechuic acid, showing that 62.5% were active against two strains of T. rubrum (Tr1 e Tr2), T. rubrum MYA 3108 (Tr3) and clinical isolate T.interdigitale (Tm1) and 68.7% for clinical isolate of T. interdigitale (Tm2) and T. interdigitale ATCC 40131 (Tm3) of the 16 compounds tested. Reference values of Minimum Inhibitory Concentration (MIC) were considered ≤ 62.5 ug/ mL. The combination of fluconazole plus pentyl, hexyl, heptyl, octyl and nonyl protocatechuates was additive to clinical isolates Tr1, Tm1 and Tm3, except the combination fluconazole plus heptyl protocatechuate, which showed a synergistic effect on Tm3. Griseofulvin in combination with nonyl protocatechuate exhibited synergistic activity two towards dermatophyte strains, additive interaction for four strains. When eight protocatechuic acid derivatives were chemically associated with miconazole, were observed synergistic and additive effects. We tested the association between protocatechuates and nystatin, resulting in antagonistic interactions. Thus it demonstrated that have not a polyene antifungal best when... (Complete abstract click electronic access below)
There is a clear need for new antifungal compounds, and the importance of natural products in the development of novel therapeutic tools. In this study, it was evaluated the antifungal activity of protocatechuic acid esters. All of the fungus isolated showed good results when testing susceptibility to synthetic derivatives of protocatechuic acid, showing that 62.5% were active against two strains of T. rubrum (Tr1 e Tr2), T. rubrum MYA 3108 (Tr3) and clinical isolate T.interdigitale (Tm1) and 68.7% for clinical isolate of T. interdigitale (Tm2) and T. interdigitale ATCC 40131 (Tm3) of the 16 compounds tested. Reference values of Minimum Inhibitory Concentration (MIC) were considered ≤ 62.5 ug/ mL. The combination of fluconazole plus pentyl, hexyl, heptyl, octyl and nonyl protocatechuates was additive to clinical isolates Tr1, Tm1 and Tm3, except the combination fluconazole plus heptyl protocatechuate, which showed a synergistic effect on Tm3. Griseofulvin in combination with nonyl protocatechuate exhibited synergistic activity two towards dermatophyte strains, additive interaction for four strains. When eight protocatechuic acid derivatives were chemically associated with miconazole, were observed synergistic and additive effects. We tested the association between protocatechuates and nystatin, resulting in antagonistic interactions. Thus it demonstrated that have not a polyene antifungal best when... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Atividade antifúngica, Eletroforese bidimensional, Antifúngicos, Sinergismo, Antifungal, Protocatechuic acid
Como citar
SOARES, Luciana Arantes. Estudo da atividade antidermatofitica de Protocatecuatos contra T. rubrum e T. interdigitale. 2011. 85 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, 2011.